Mouse anti-Human TREM-1 Monoclonal Antibody | anti-TREM-1 antibody

TREM-1, Human, mAb 6B1

Cat. Num. AAA584178

• Gene Names: TREM1; CD354; TREM-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Protein G
• Synonyms: TREM-1; Monoclonal Antibody; Human; mAb 6B1; triggering receptor expressed on myeloid cells 1; TREM1; anti-TREM-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Protein G
• Form/Format: 0.2 um filtered in PBS+0.1%BSA; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Concentration: 100 ug/ml (varies by lot)
• Sequence Length: 225

• Applicable Applications for anti-TREM-1 antibody: Flow Cytometry (FC/FACS), Functional Studies (FS), Immunoassays (IA), Western Blot (WB)
• Application Notes: Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting.
• Conjugate: None
• Immunogen: Recombinant TREM-1 - IgG1 fusion protein.
• Preparation and Storage: Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.

Related Product Information for anti-TREM-1 antibody

The monoclonal antibody 6B1 recognizes human triggering receptor expressed on myeloid cells 1 (TREM-1). TREM-1 is a transmembrane glycoprotein belonging to a family related to the natural killer cell receptors. TREM-1 is an important activating receptor, of 26 kDa, involved in the innate inflammatory response and in sepsis. TREM-1 is expressed at low levels in the early development of the hematopoietic system and in the promonoistic stage and at high levels on neutrophil granulocytes (PMN), monocytes, and macrophage subsets. The expression of TREM-1 is upregulated by microbial products, that is, by toll-like receptor ligands such as lipoteichoic acid (LTA) of Gram-positive or lipopolysaccharide (LPS) of Gram-negative bacteria. Ligation of TREM-1 is synergistic with TLR agonists on the activation of receptor bearing cells. Platelets express a natural ligand for TREM-1. Receptor ligation activates the full repertoire of effector functions. TREM-1 is also produced in a soluble form (sTREM-1) of 17 kDa which is released in humans after endotoxin exposition or in patients suffering from severe pneumonia or sepsis. sTREM-1 can be measured in biological fluids and may be useful as a tool. The monoclonal antibody 6B1 recognizes both membrane bound and soluble TREM-1. Also, the monoclonal antibody 6B1 neutralizes the interaction between TREM-1 and its ligand.

References

1. Radsak, M et al; Soluble triggering receptor expressed on myeloid cells 1 is released in patients with stable chronic obstructive pulmonary disease. Clin Dev Immunol 2007, 2007: 52040 2. Haselmayer, P et al; TREM-1 ligand expression on platelets enhances neutrophil activation. Blood 2007, 110: 1029

NCBI and Uniprot Product Information

• NCBI GI #: 336088680
• NCBI GeneID: 54210
• NCBI Accession #: NP_001229518.1
• NCBI GenBank Nucleotide #: NM_001242589.1
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 1 isoform 2
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 1
• NCBI Official Symbol: TREM1
• NCBI Official Synonym Symbols: CD354; TREM-1
• NCBI Protein Information: triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 1
• UniProt Gene Name: TREM1
• UniProt Synonym Gene Names: TREM-1
• UniProt Entry Name: TREM1_HUMAN

NCBI Description

This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

TREM1: Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; integral to membrane; plasma membrane

Molecular Function: receptor activity

Biological Process: neutrophil chemotaxis; innate immune response; cytokine secretion during immune response; blood coagulation; leukocyte migration; chemokine metabolic process; humoral immune response