Mouse TRACP5a Monoclonal Antibody | anti-TRACP5a antibody
TRACP5a, Human, mAb 220
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein G
Synonyms
TRACP5a; Monoclonal Antibody; Human; mAb 220; TRAP5; anti-TRACP5a antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
220
Purity/Purification
Protein G
Form/Format
0.2 um filtered in PBS, +0.1% bovine serum albumin +0.02% NaN3.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Applicable Applications for anti-TRACP5a antibody
Immuno Assay (IA), Immunoprecipitation (IPP), Immunohistochemistery Paraffin sections (IHC-P), Western Blot (WB)
Application Notes
W: A non-reduced and reduced sample treatment and SDS-Page was used. The band sizes are 14 and 35 kDa (Ref.1).
IHC-P: Tissue sections were pretreated with prior heat-induced antigen retrieval by incubation overnight at 72 degree C in a commercial solution of EDTA at pH 8.0.
Positive control: Granular cell tumor of the skin and ductal carcinoma of the breast
Negative control: Mouse IgG at 1 ug/mL (Western Blot).
IHC-P: Tissue sections were pretreated with prior heat-induced antigen retrieval by incubation overnight at 72 degree C in a commercial solution of EDTA at pH 8.0.
Positive control: Granular cell tumor of the skin and ductal carcinoma of the breast
Negative control: Mouse IgG at 1 ug/mL (Western Blot).
Immunogen
Recombinant TRACP
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year.
Related Product Information for anti-TRACP5a antibody
Monoclonal antibody 220 recognizes human tartrate-resistant-acid phosphatase 5a (TRACP5a). TRACP is considered a marker for differentiated monocytes like macrophages, dendritic cells and osteoclasts. It is also considered to be a serum marker for bone disease. TRACP has two isotypes (5a &5b) with different characteristics. Isoform 5a is intact and bears a sialic acid and has a lower activity and pH optimum (pH5.0-5.2) than TRACP5b (pH5.8-6.0). TRACP5a circulates as a 35kD glycoprotein and 5b as a linked heterodimer of proteolytically cleaved16 & 23 kDa fragments. TRACP5a has unique epitopes associated with the intact loop peptide. Antibody 220 recognizes the regulatory loop peptide which interacts with the active site of TRACP5a. Serum TRACP protein is predominantly isoform 5a, while most of the activity is due to isoform 5b. TRACP5a, but not 5b is secreted by activated macrophages and dendritic cells and is therefore considered a marker for systemic macrophage and chronic inflammation. TRACP 5a levels are correlated positively with inflammation-related markers CRP, ferritin, and triglycerides, which are also elevated in cardiovascular patients (CVD), but have no relationship to TRAP 5b activity or other bone metabolic markers. TRACP5a is a specific secreted marker of inflammatory macrophages relevant to their systemic number. TRACP5b is a marker for osteoclast number and bone resorption. The clinical significance of TRACP5a is not completely clear yet. TRACP5a levels are elevated in end stage renal disease, 30% of rheumatoid arthritis patients and was positively correlated with body mass and fat indices (adipose tissue macrophages). Elevated serum TRACP5a may be an indicator of advanced or systemic inflammatory disease associated with inflammatory vulnerable plaque and a risk marker for adverse events in CVD.
References
Pradella, S et al; Applications and performance of monoclonal antibodies to human tartrate resistant acid phosphatase. J Immunol Methods. 2011 Sep 30;372(1-2):162-70
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Product Notes
The TRACP5a (Catalog #AAA584664) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TRACP5a can be used in a range of immunoassay formats including, but not limited to, Immuno Assay (IA), Immunoprecipitation (IPP), Immunohistochemistery Paraffin sections (IHC-P), Western Blot (WB). W: A non-reduced and reduced sample treatment and SDS-Page was used. The band sizes are 14 and 35 kDa (Ref.1). IHC-P: Tissue sections were pretreated with prior heat-induced antigen retrieval by incubation overnight at 72 degree C in a commercial solution of EDTA at pH 8.0. Positive control: Granular cell tumor of the skin and ductal carcinoma of the breast Negative control: Mouse IgG at 1 ug/mL (Western Blot). . Researchers should empirically determine the suitability of the TRACP5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRACP5a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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