Immunocytochemistry (ICC)
(Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.)
Mouse TPX2 Monoclonal Antibody | anti-TPX2 antibody
Anti-TPX2 Purified
Gene Names
TPX2; DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
Reactivity
Rat, Mouse, Human
Applications
Immunoprecipitation, Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA
Purity
Purified by Protein-A Affinity Chromatography
Synonyms
TPX2; Monoclonal Antibody; Anti-TPX2 Purified; TPX2 microtubule nucleation factor; DIL2; HCTP4; REPP86; anti-TPX2 antibody
Host
Mouse
Reactivity
Rat, Mouse, Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
TPX2-01
Specificity
The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
Purity/Purification
Purified by Protein-A Affinity Chromatography
Form/Format
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Concentration
1 mg/ml (varies by lot)
Sequence Length
747
Applicable Applications for anti-TPX2 antibody
Immunoprecipitation, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), ELISA (EIA)
Immunogen
Human TPX2
Preparation and Storage
Store at 2-8 degree C.
Immunocytochemistry (ICC)
(Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.)
Immunocytochemistry (ICC)
(Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.)
Related Product Information for anti-TPX2 antibody
TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
targeting protein for Xklp2
NCBI Official Synonym Full Names
TPX2 microtubule nucleation factor
NCBI Official Symbol
TPX2
NCBI Official Synonym Symbols
DIL2; p100; DIL-2; HCTP4; FLS353; HCA519; REPP86; C20orf1; C20orf2; GD:C20orf1
NCBI Protein Information
targeting protein for Xklp2
UniProt Protein Name
Targeting protein for Xklp2
Protein Family
UniProt Gene Name
TPX2
UniProt Synonym Gene Names
C20orf1; C20orf2; DIL2; HCA519; DIL-2; p100
UniProt Entry Name
TPX2_HUMAN
Uniprot Description
TPX2: a microtubule-associated protein required for meiotic and mitotic spindle assembly. Targets a plus end-directed motor (XKLP2) to the minus ends of microtubules in the mitotic spindle. Required for microtubule nucleation around chromosomes in response to the local generation of RanGTP. Binds to and activates Aurora A kinase and targets it to the mitotic spindle.
Protein type: Microtubule-binding; Cell cycle regulation
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: nucleoplasm; microtubule cytoskeleton; axon hillock; spindle pole; microtubule; cytoplasm; spindle; nucleus
Molecular Function: protein binding; GTP binding; protein kinase binding; ATP binding
Biological Process: mitosis; cell proliferation; apoptosis; cell division; activation of protein kinase activity
Research Articles on TPX2
Similar Products
Product Notes
The TPX2 tpx2 (Catalog #AAA1801591) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-TPX2 Purified reacts with Rat, Mouse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPX2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), ELISA (EIA). Researchers should empirically determine the suitability of the TPX2 tpx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPX2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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