Western Blot (WB)
(TOP2A Antibody western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TOP2A antibody detected the TOP2A protein (arrow).)
Mouse anti-Human TOP2A Monoclonal Antibody | anti-TOP2A antibody
TOP2A Antibody
Western Blot (WB)
(TOP2A Antibody western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TOP2A antibody detected the TOP2A protein (arrow).)
Western Blot (WB)
(TOP2A Antibody western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TOP2A antibody detected the TOP2A protein (arrow).)
controls and alters the topologic states of DNA during
transcription. This nuclear enzyme is involved in processes such as
chromosome condensation, chromatid separation, and the relief of
torsional stress that occurs during DNA transcription and
replication. It catalyzes the transient breaking and rejoining of
two strands of duplex DNA which allows the strands to pass through
one another, thus altering the topology of DNA. Two forms of this
enzyme exist as likely products of a gene duplication event. The
gene encoding this form, alpha, is localized to chromosome 17 and
the beta gene is localized to chromosome 3. The gene encoding this
enzyme functions as the target for several anticancer agents and a
variety of mutations in this gene have been associated with the
development of drug resistance. Reduced activity of this enzyme may
also play a role in ataxia-telangiectasia.
Chen, H., et al. Am. J. Surg. Pathol. 34(9):1250-1257(2010)
Ye, J., et al. Cell 142(2):230-242(2010)
Rossi, E., et al. Histopathology 57(1):81-89(2010)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Uniprot Description
TOP2A: a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described.
Protein type: EC 5.99.1.3; Isomerase
Chromosomal Location of Human Ortholog: 17q21-q22
Cellular Component: centriole; nucleoplasm; nuclear chromosome; protein complex; DNA topoisomerase complex (ATP-hydrolyzing); viral integration complex; nucleolus; condensed chromosome; nucleus
Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; DNA topoisomerase (ATP-hydrolyzing) activity; ubiquitin binding; protein homodimerization activity; histone deacetylase binding; magnesium ion binding; drug binding; protein binding; enzyme binding; DNA binding; protein kinase C binding; protein heterodimerization activity; chromatin binding; DNA bending activity; ATP binding
Biological Process: DNA unwinding during replication; mitotic recombination; positive regulation of viral genome replication; DNA topological change; positive regulation of apoptosis; rhythmic process; sister chromatid segregation; apoptotic chromosome condensation; regulation of circadian rhythm; embryonic cleavage; positive regulation of retroviral genome replication; DNA ligation; chromosome segregation; resolution of meiotic joint molecules as recombinants; hemopoietic progenitor cell differentiation; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; response to DNA damage stimulus
Research Articles on TOP2A
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Product Notes
The TOP2A top2a (Catalog #AAA9200506) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TOP2A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOP2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:500~8000. Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP2A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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