Mouse anti-Human, Rat TNF-RII Monoclonal Antibody | anti-TNF-RII antibody
TNF-RII, Human, mAb 80M2, biotinylated
Reactivity
Human, Rat
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Synonyms
TNF-RII; Monoclonal Antibody; Human; mAb 80M2; biotinylated; Tumor necrosis factor receptor superfamily member 1B; Tumor necrosis factor receptor 2; CD120b; TNF-R2; p75; p80; Tumor necrosis factor receptor type II; TNF-alpha receptorGene name: TNFRSF1B; TNFBR; TNFR2; TNF-RII Human mAb 80M2 Biotin 50ug; anti-TNF-RII antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Form/Format
0.5ml (100ug/ml) 0.2um filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
Applicable Applications for anti-TNF-RII antibody
Flow Cytometry (FC/FACS), Functional Studies (FS), Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
The typical starting working dilution is 1:50.
FC: Antibody 80M2 was used to select HeLa cells expressing TNF-RII- by- FACS. As negative control mock transfected HeLa cells were used. (Ref.2)
FS: Antibody 80M2 functions as an allosteric modulator, which stabilizes the ligand-receptor complex. (Ref.1)
FC: Antibody 80M2 was used to select HeLa cells expressing TNF-RII- by- FACS. As negative control mock transfected HeLa cells were used. (Ref.2)
FS: Antibody 80M2 functions as an allosteric modulator, which stabilizes the ligand-receptor complex. (Ref.1)
Conjugation
Biotin
Positive Control
HeLa cells- transfected with TNF-RII
Negative Control
Mock transfected HeLa cells
Preparation and Storage
Product should be stored at 4 degree C.
Under recommended storage conditions, product is stable for at least one year.
Under recommended storage conditions, product is stable for at least one year.
Related Product Information for anti-TNF-RII antibody
The monoclonal antibody 80M2 recognizes the extracellular part of- membrane-bound TNF-RII as well as- the soluble form of TNF-RII which is generated by proteolytic cleavage of the extracellular domain. The soluble form can still bind TNF-alpha with high affinity and functions as a TNF-alpha antagonist.- TNF-alpha is an important signaling protein in the immune system which can activate inflammatory responses, induce apoptosis, regulate cellular proliferation, and may even promote cancer progression. TNF-alpha can bind to two structurally distinct membrane receptors, TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. TNFRI is believed to be expressed on nearly all cell types, whereas TNFRII exhibits more restricted expression, being found on certain subpopulations of immune cells and several other cell types. A dominant role of TNF-RII has been shown in thymocyte activation by TNF-alpha, whereas induction of cytotoxicity and other functions are mediated largely by TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is activated only by the membrane bound form of TNF-alpha. The antibody is a non-agonistic receptor modulating antibody. It enhances in vitro TNF alpha responses by increasing the affinity of the soluble form of TNF-alpha for TNF-RII.
Product Categories/Family for anti-TNF-RII antibody
References
1. Grell, M et al; The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor. Cell 1995, 83: 793
2. Till, A et al; The Met 196 - Arg variation ofHuman tumor necrosis factor receptor 2 (TNFR2) affects TNF-alpha induced apoptosis by impaired NF-kB signalling and target gene expression. J Biol Chem 2005, 280: 5994
3. Scherubl C. et al; Colocalization of endogenous TNF with a functional intracellular splice form ofHuman TNF receptor type 2. Journal of Inflammation 2005, 2: 7
4. Krippner, A et al; Single chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
5. Fairfax, B et al; A common haplotype of the TNF receptor 2 gene modulates endotoxin tolerance. J. Immonol 2011, 186: 3058
2. Till, A et al; The Met 196 - Arg variation ofHuman tumor necrosis factor receptor 2 (TNFR2) affects TNF-alpha induced apoptosis by impaired NF-kB signalling and target gene expression. J Biol Chem 2005, 280: 5994
3. Scherubl C. et al; Colocalization of endogenous TNF with a functional intracellular splice form ofHuman TNF receptor type 2. Journal of Inflammation 2005, 2: 7
4. Krippner, A et al; Single chain TNF, a TNF derivative with enhanced stability and antitumoral activity. J Immunol 2008, 180: 8176
5. Fairfax, B et al; A common haplotype of the TNF receptor 2 gene modulates endotoxin tolerance. J. Immonol 2011, 186: 3058
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Product Notes
The TNF-RII (Catalog #AAA584862) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TNF-RII, Human, mAb 80M2, biotinylated reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNF-RII can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Functional Studies (FS), Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP). The typical starting working dilution is 1:50. FC: Antibody 80M2 was used to select HeLa cells expressing TNF-RII- by- FACS. As negative control mock transfected HeLa cells were used. (Ref.2) FS: Antibody 80M2 functions as an allosteric modulator, which stabilizes the ligand-receptor complex. (Ref.1). Researchers should empirically determine the suitability of the TNF-RII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNF-RII, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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