anti-Mouse TNF-RI Monoclonal Antibody | anti-TNF-RI antibody

TNF-RI, Mouse, mAb 55R-170

Cat. Num. AAA584105

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Synonyms: TNF-RI; Monoclonal Antibody; Mouse; mAb 55R-170; TNFR type I; CD120a; TNF-R55; TNFRp55; p55-R; TNF receptor alpha chain; anti-TNF-RI antibody

• Host: Armenian hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian hamster IgG
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 471

• Applicable Applications for anti-TNF-RI antibody: Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunoprecipitation (IP)
• Application Notes: Application Use: For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
• Immunogen: Purified soluble extracellular domain of mouse TNF-RI
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TNF-RI antibody

Product Description: The monoclonal antibody 55R-170 recognizes the extracellular part of mouse Tumor Necrosis Factor Receptor superfamily member 1A (TNF-RI), also known as CD120a or p55. TNF-RI belongs to the large TNF receptor family, among which TNF-RII (TNF-R p75-80), lymphotoxin-beta receptor (LTbetaR) and the Herpes virus entry mediator (HVEM). Ligands for these receptors belong to the Tumor Necrosis Factor (TNF) superfamily of cytokines, which activate signaling pathways for cell survival, death, and differentiation that orchestrate the development, organization and homeostasis of lymphoid, mammary, neuronal and ectodermal tissues. TNF-RI contains a characteristic structural cassette termed death domain in its sequence that is conserved within a distinct subset of other TNF-R family members, such as CD95, DR3, DR4, and DR5. This death domain, was characterized as being essential for induction of apoptosis in vitro and has been structurally conserved within these TNF-R superfamily members. Deletion of the death domain of the TNF-RI results in a non-functional receptor, indicating that the death domain is required for the signal transduction of the physiological functions of TNF-RI in vivo. TNF-RI is a 55 kD type I transmembrane protein and is expressed on a variety of cell types at low levels. It is considered to play a prominent role in cell stimulation by TNF-alpha. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is present as soluble form in body fluids (for instance plasma and CSF). This extracellular TNF-RI is generated by two mechanisms, namely proteolytic cleavage of TNF-RI ectodomains and release of full-length TNF-RI in the membranes of exosome-like vesicles. TNF-RI and TNF-RII both interact with the homomeric forms of LTbeta or TNF. However, TNF-RI functions as the high affinity receptor for soluble TNF (sTNF). TNF-RI has been shown to be involved in a wide variety of inflammatory diseases, among which neurodegenerative diseases (Parkinson's and Alzheimer's disease), multiple sclerosis, asthma, atherosclerosis, rheumatology. The monoclonal antibody 55R-170 also recognizes the soluble receptor..

References

1. Sheehan, K et al; Monoclonal antibodies specific for murine p55 and p75 tumor necrosis factor receptors: identification of a novel in vivo role for p75. J Exp Med 1995, 181: 607 2. Pinckard, J et al; Ligand-induced formation of p55 and p75 tumor necrosis factor receptor heterocomplexes on intact cells. J Biol Chem 1997, 272: 10784
3. Ji, H et al; Critical roles for interleuking 1 and tumor necrosis factor alpha in antibody-induced arthritis. J Exp Med 2002, 196: 77
4. Lee, S et al; Inhibition of TCR-induced CD8 T cell death by IL-12: regulation of Fas ligand and cellular FLIP expression and caspase activation by IL-12. J Immunol 2003, 170: 2456

NCBI and Uniprot Product Information

• NCBI GI #: 2290398
• NCBI GeneID: 282527
• Molecular Weight: 51,368 Da
• NCBI Official Full Name: tumor necrosis factor-receptor I
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1A<
• NCBI Official Symbol: TNFRSF1A
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A; p55; p60; TNF-R1; TNF-RI; TNFR-I; tumor necrosis factor receptor 1; tumor necrosis factor receptor type I
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• UniProt Gene Name: TNFRSF1A
• UniProt Synonym Gene Names: TNFR1; TNF-R1; TNF-RI; TNFR-I
• UniProt Entry Name: TNR1A_BOVIN

Uniprot Description

Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis ().