Mouse anti-Human TNF alpha Monoclonal Antibody | anti-TNF alpha antibody

anti-human TNF alpha-Purified (with Antibiotics)

Cat. Num. AAA666533

• Gene Names: TNF; DIF; TNFA; TNFSF2; TNF-alpha
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Western Blot
• Purity: Purified (with Antibiotics)
• Synonyms: TNF alpha; Monoclonal Antibody; anti-human TNF alpha-Purified (with Antibiotics); Monoclonal anti-human TNF alpha; anti-TNF alpha antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: J1D9
• Purity/Purification: Purified (with Antibiotics)
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5% Gentamicin sulfate
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 233

• Applicable Applications for anti-TNF alpha antibody: Flow Cytometry (FC/FACS), Western Blot (WB), FX
• Performance: Ficoll prepared human peripheral blood mononuclear cells were stimulated by culturing 6 hours in the presence of 50 ng/ml PMA, 1 muM Ionomycin and 1 mug/ml Brefeldin A. Cells were then harvested, washed and fixed for 30 min with 2% buffered formaldehyde after which they were washed two times. Fixed cells were permeabolized 10 minutes in a buffer containing 0.3% Saponin. Subsequent reagent incubations and washes were done using this buffer. Five x 105 cells per tube were pre incubated 5 minutes with 20 mul of 250 mug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-TNFalpha antibody at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A 20% sub population of the cells stained positive with a mean shift of 0.89 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
• Group Header: anti-human TNF alpha
• Immunogen: Recombinant human TNF alpha
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-TNF alpha antibody

Information: Human tumor necrosis factor alpha (TNF alpha) is a proinflammatory cytokine expressed mainly by activated monocytes and macrophages. TNF alpha exerts a key role with regard to pathogenesis of many infectious and inflammatory diseases. Antibody J1D9 neutralizes TNF alpha biological activities (2).

References

1) P. Vassalli, (1992) Annu Rev Immunol 10: 411-452. 2) P.J. McLaughlin, et al, (1992) Anticancer Res 12: 1243-1246. 3) M. Pasparakis, et al, (1996) Cytokine and Growth Factor Reviews 7: 223-229. 4) A. Eigler, et al, (1997) Immunology Today 18: 487-492.

NCBI and Uniprot Product Information

• NCBI GI #: 37210
• NCBI GeneID: 7124
• UniProt Accession #: P01375; O43647; Q9P1Q2; Q9UIV3
• Molecular Weight: 25,644 Da
• NCBI Official Full Name: TNF-alpha
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: DIF; TNFA; TNFSF2; TNF-alpha
• NCBI Protein Information: tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_HUMAN

NCBI Description

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]

Uniprot Description

TNF-a: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Homotrimer. Interacts with SPPL2B. Belongs to the tumor necrosis factor family.

Protein type: Cytokine; Apoptosis; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; recycling endosome; cell surface; integral to plasma membrane; plasma membrane; extracellular region; external side of plasma membrane; lipid raft; phagocytic cup

Molecular Function: identical protein binding; protein binding; protease binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: positive regulation of JNK activity; extracellular matrix organization and biogenesis; positive regulation of nitric oxide biosynthetic process; positive regulation of NFAT protein import into nucleus; positive regulation of osteoclast differentiation; activation of MAPK activity; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; positive regulation of caspase activity; positive regulation of NF-kappaB import into nucleus; osteoclast differentiation; positive regulation of translational initiation by iron; positive regulation of membrane protein ectodomain proteolysis; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; negative regulation of interleukin-6 production; JNK cascade; negative regulation of osteoblast differentiation; positive regulation of action potential; regulation of immunoglobulin secretion; embryonic gut development; negative regulation of protein complex disassembly; positive regulation of cytokine production; response to drug; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of heterotypic cell-cell adhesion; positive regulation of mitosis; response to virus; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; glucose metabolic process; negative regulation of fat cell differentiation; positive regulation of chemokine production; negative regulation of cytokine secretion during immune response; positive regulation of protein transport; detection of mechanical stimulus involved in sensory perception of pain; cell activation; defense response to Gram-positive bacterium; induction of apoptosis via death domain receptors; DNA damage response, signal transduction resulting in induction of apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to activity; negative regulation of L-glutamate transport; negative regulation of transcription, DNA-dependent; skeletal muscle contraction; sequestering of triacylglycerol; positive regulation of smooth muscle cell proliferation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-18 production; chronic inflammatory response to antigenic stimulus; response to salt stress; positive regulation of synaptic transmission; positive regulation of hair follicle development; negative regulation of cell proliferation; response to radiation; negative regulation of lipid catabolic process; positive regulation of neuron apoptosis; protein kinase B signaling cascade; lipopolysaccharide-mediated signaling pathway; positive regulation of chronic inflammatory response to antigenic stimulus; inflammatory response; regulation of I-kappaB kinase/NF-kappaB cascade; caspase activation; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of protein complex disassembly; transformed cell apoptosis; MAPKKK cascade; calcium-mediated signaling; positive regulation of peptidyl-serine phosphorylation; humoral immune response; positive regulation of protein kinase B signaling cascade; positive regulation of interferon-gamma production; positive regulation of programmed cell death; negative regulation of glucose import; positive regulation of chemokine biosynthetic process; positive regulation of protein complex assembly; negative regulation of viral genome replication; protein import into nucleus, translocation; positive regulation of protein kinase activity; response to hypoxia; positive regulation of fever; activation of MAPKKK activity; receptor biosynthetic process; positive regulation of protein amino acid phosphorylation; negative regulation of myoblast differentiation; leukocyte tethering or rolling; regulation of insulin secretion; positive regulation of cytokine secretion

Disease: Asthma, Susceptibility To; Migraine With Or Without Aura, Susceptibility To, 1; Malaria, Susceptibility To