Mouse TLR9 Monoclonal Antibody | anti-TLR9 antibody

TLR9, Mouse, mAb 5G5, biotinylated

Cat. Num. AAA584853

• Gene Names: TLR9; CD289
• Reactivity: Mouse, Canine,Human
• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
• Synonyms: TLR9; Monoclonal Antibody; Mouse; mAb 5G5; biotinylated; TLR9 Mouse mAb 5G5 Biotin 50ug; anti-TLR9 antibody

• Host: Mouse
• Reactivity: Mouse, Canine,Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Form/Format: 0.5ml (100ug/ml) 0.2um filtered biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
• Sequence Length: 1031

• Applicable Applications for anti-TLR9 antibody: Immunoassay (IA), Western Blot (WB), Immunohistochemistry (IHC) Frozen, Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: The typical starting working dilution is 1:50.; The monoclonal antibody 5G5 can be used for flow cytometry, Western blotting and immuno assays as detection antibody. Furthermore, the monoclonal antibody 5G5 is useful for immunohistology on frozen and paraffin sections. The monoclonal antibody 5G5 stains RAW macrophages and TLR9 (CD289) transfected HEK293 cells. The antibody is weakly cross reactive withHuman TLR9.
• Conjugation: Biotin
• Preparation and Storage: Product should be stored at 4 degree C.; Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR9 antibody

The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and is cross reactive with canine TLR9.

Product Categories/Family for anti-TLR9 antibody

Infectious diseases

References

1. Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958
2. Rutz, M et al; Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pH-dependent manner. Eur J Immunol 2004, 34: 2541
3. Burgener, I et al; Antibodies specific forHuman or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184

NCBI and Uniprot Product Information

• NCBI GI #: 229473723
• NCBI GeneID: 54106
• NCBI Official Full Name: TLR9, partial
• NCBI Official Synonym Full Names: toll like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• Protein Family: Toll-like receptor

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Aug 2017]