Mouse TLR9 Monoclonal Antibody | anti-TLR9 antibody

TLR9, Human, mAb 5G5

Cat. Num. AAA584459

• Gene Names: TLR9; CD289
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: TLR9; Monoclonal Antibody; Human; mAb 5G5; CD289; anti-TLR9 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Form/Format: 1.0 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 1032

• Applicable Applications for anti-TLR9 antibody: F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunofluorescence (IF), P, Western Blot (WB)
• Application Notes: Application Notes: F: 10 um sections were fixed with acetone for 10 minutes. PBS washed sections were incubated with 5G5 1:100 in 1% BSA for 30 minutes at RT. (Ref.7); Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Immunogen: Purified- fusion protein of extracellular domain of human TLR9 (AA 1-815) and human IgGFc
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR9 antibody

Product Description: The monoclonal antibody 5G5 recognizes human Toll-like receptor 9. Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called ˜pathogen-associated molecular patterns. Activation of TLRs, a family of at least 11 differentmembers that function either as homo- or heterodimers, leads to activation of NF degree B-dependent and IFNregulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens.
Whereas most TLRs are expressed on the cell surface, TLR9 is expressed intracellularly within one or more endosomal compartments and recognizes nucleic acids. TLR9 detects a rather subtle difference in the DNA of vertebrates compared with that of pathogens. Vertebrate genomic DNAs have mostly methylated CpG dinucleotides where bacterial and viral DNAs have unmethylated CpG dinucleotides. TLR9 undergoes relocation from endoplasmic reticulum to CpG-ODN-containing endosomes. In these endosomes TLR9 becomes a functional receptor after proteolytic cleavage. TLR9 exists as a preformed homodimer and CpG-ODN binding promotes its conformational change, bringing the cytoplasmic TIR-like domains close to each other. This allows a recruitment of the key adapter protein MyD88 which initiates a signalling cascade. The only human immune cell types known to constitutively express TLR9 and to be activated by CpG ODN are pDCs and B cells. TLR9 triggering induces an activation phenotype in the B cells and pDCs, characterized by the expression of costimulatory molecules, resistance to apoptosis, and induces Th1-type immune response profiles.

References

1. Ahmad-Nejad, P et al; Bacterial CpG-DNA and lipopolysaccharides activate Toll-like receptors at distinct cellular compartments. Eur J Immunol 2002, 32: 1958
2. Rutz, M et al; Toll-like receptor 9 binds single-stranded CpG-DNA in a sequence- and pHdependent manner. Eur J Immunol 2004, 34: 2541
3. Rumio C et al; Degranulation of Paneth cells via Toll-like receptor 9. Am J Pathol 2004, 165:373
4. Pratesi, G et al; Therapeutic synergism of gemcitabine and CpG-oligodeoxynucleotides in an orthotopic human pancreatic carcinoma xenograft. Cancer res 2005, 65: 6388
5. Tokumasa, N et al; Expression of Tyk2 in dendritic cells is required for Il-2, Il-23, and IFNg production and the induction of Th1 cell differentiation. Blood 2007, 110: 553
6. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184
7. Machida, H et al; Expression of Toll-like receptor 9 in renal podocytes in childhood-onset active and inactive lupus nephritis. Nephrol Dial Transplant 2010, 25: 2530
8. Zheng, W et al. Distinct host-related dendritic cell responses during the early stage of Plasmodium yoelii infection in susceptible and resistant mice. Parasite immunology 2010, 32: 324

NCBI and Uniprot Product Information

• NCBI GI #: 74054321
• NCBI GeneID: 54106
• Molecular Weight: 114,237 Da
• NCBI Official Full Name: TLR9
• NCBI Official Synonym Full Names: toll-like receptor 9
• NCBI Official Symbol: TLR9
• NCBI Official Synonym Symbols: CD289
• NCBI Protein Information: toll-like receptor 9
• UniProt Protein Name: Toll-like receptor 9
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR9
• UniProt Synonym Gene Names: UNQ5798/PRO19605
• UniProt Entry Name: TLR9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR9: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Interacts with MYD88 via their respective TIR domains. Interacts (via transmembrane domain) with UNC93B1. Interacts with CD300LH; the interaction may promote full activation of TLR9-triggered innate responses. Interacts with BTK. Interacts with CNPY3 and HSP90B1; this interaction is required for proper folding in the endoplasmic reticulum. Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre- dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver. Belongs to the Toll-like receptor family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; lysosome; endoplasmic reticulum; basolateral plasma membrane; apical plasma membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane; endosome membrane; endosome

Molecular Function: siRNA binding; transmembrane receptor activity; interleukin-1 receptor binding

Biological Process: positive regulation of JNK activity; regulation of cytokine secretion; positive regulation of interleukin-12 production; maintenance of gastrointestinal epithelium; positive regulation of JNK cascade; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-18 production; defense response to Gram-negative bacterium; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to molecule of bacterial origin; negative regulation of interleukin-8 production; positive regulation of interferon-beta production; negative regulation of interleukin-6 production; inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; negative regulation of toll-like receptor signaling pathway; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response