anti-Human TLR7 Monoclonal Antibody | anti-TLR7 antibody

TLR7 Monoclonal Antibody

Cat. Num. AAA668010

• Gene Names: TLR7; TLR7-like
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry
• Purity: Purified; Protein G Chromatography
• Synonyms: TLR7; Monoclonal Antibody; TLR7 Monoclonal Antibody; anti-TLR7 antibody

• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, Kappa
• Clone Number: ABM2C27
• Purity/Purification: Purified; Protein G Chromatography
• Form/Format: 25ug in 50 ul/100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
• Concentration: 0.5 mg/ml, 200 µl (varies by lot)
• Sequence Length: 1049

• Applicable Applications for anti-TLR7 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC)
• Application Notes: FACS analysis: 0.5 ug/10⁶ cells, Immunohistochemical analysis: 5ug/ml
• Antigen: TLR7
• Immunogen: A partial recombinant human TLR7 protein (amino acids 500-900) was used as the immunogen for the antibody
• Preparation and Storage: Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western Blot (WB)

(Expression analysis of TLR7. Anti- TLR7 antibody (Clone: ABM2C27) was used at 2 µg/ml on Ramos lysate.)

Flow Cytometry (FC/FACS)

(Intracellular flow analysis of TLR7 in PBMC (Monocyte) using 0.5 µg/10^6 cells of TLR7 antibody (Clone: ABM2C27). Green represents isotype control; red represents anti-TLR7 antibody. Goat anti-mouse PE conjugate was used as secondary.)

Flow Cytometry (FC/FACS) #2

(Intracellular flow analysis of TLR7 in Raji using 0.5 µg/10^6 cells of TLR7 antibody (Clone: ABM2C27). Green represents isotype control; red represents anti-TLR7 antibody. Goat anti-mouse PE conjugate was used as secondary.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of TLR7 in normal human Kidney tissue using TLR7 antibody (Clone: ABM2C27) at 5 ug/ml.)

Immunohistochemistry (IHC) #2

(Immunohistochemical analysis of TLR7 in Renal Cell Carcinoma using TLR7 antibody (Clone: ABM2C27) at 5 ug/ml.)

Related Product Information for anti-TLR7 antibody

TLR7 (Toll-like receptor 7) is a member of the Toll-like receptor (TLR) family. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA; which plays a fundamental role in pathogen recognition and activation of innate immunity. TLR7 controls host immune response against pathogens through recognition of molecular patterns specific to microorganisims. TLR7 interacts with MYD88 via their respective TIR domains and also interacts with UNC93B1. Post-translational modifications and glycosylation occur in TLR7. TLR7 detected in brain, placenta, spleen, stomach, small intestine, lungs and plasmacytoid pre-dendritic cells.

Product Categories/Family for anti-TLR7 antibody

TLR

NCBI and Uniprot Product Information

• NCBI GI #: 7706093
• NCBI GeneID: 51284
• NCBI Accession #: NP_057646.1
• NCBI GenBank Nucleotide #: NM_016562.3
• UniProt Accession #: Q9NYK1; Q9NR98; D1CS69
• NCBI Official Full Name: toll-like receptor 7
• NCBI Official Synonym Full Names: toll-like receptor 7
• NCBI Official Symbol: TLR7
• NCBI Official Synonym Symbols: TLR7-like
• NCBI Protein Information: toll-like receptor 7; toll-like receptor 7-like
• UniProt Protein Name: Toll-like receptor 7
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR7
• UniProt Entry Name: TLR7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR7: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Interacts with MYD88 via their respective TIR domains. Interacts with UNC93B1. Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; lysosome; endoplasmic reticulum; cytoplasm; plasma membrane; integral to membrane; endosome membrane; receptor complex; endosome

Molecular Function: single-stranded RNA binding; siRNA binding; transmembrane receptor activity; double-stranded RNA binding; drug binding

Biological Process: positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; microglial cell activation; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 7 signaling pathway; positive regulation of chemokine production; positive regulation of interleukin-8 production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; inflammatory response; defense response to virus; positive regulation of inflammatory response; positive regulation of interferon-gamma biosynthetic process