Mouse TLR3 Monoclonal Antibody | anti-TLR3 antibody

TLR3, Human, mAb TLR3.7

Cat. Num. AAA584129

• Gene Names: TLR3; CD283; IIAE2
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Western Blot
• Synonyms: TLR3; Monoclonal Antibody; Human; mAb TLR3.7; CD283; anti-TLR3 antibody

• Host: Mouse
• Reactivity: Reactivity: Human. Cross reactivity: Canine, Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 904

• Applicable Applications for anti-TLR3 antibody: F, Flow Cytometry (FC/FACS), FS, Immunoflurescence (IF), Immunoprecipitation (IP), P, Western Blot (WB)
• Application Notes: Application Notes: F: dried sections, fixed with 4% paraformaldehyde and subsequently washed in PBS and MQ. Sections were quenched with 0.3% H2O2 in methanol and washed in PBS. Sections were permeabilized with 0.4% triton-X100 in PBS. Pretreated slides were blocked with 1% horse serum for 20' and incubated o/n with antibody (Ref.5).; Application Use: For flow cytometry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For inhibition studies dilutions have to be made according to the amounts ligand to be inactivated.
• Immunogen: Human DYKDDDDK-tagged TLR3 stably expressed by- Ba/F3 cells
• Positive Control: Monocytes, granulocytes, lymphocytes, human fibroblast, MRC-5 & FS-4 cells
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-TLR3 antibody

Product Description: The monoclonal antibody TLR3.7 recognizes the 116 kDa human Toll-like receptor 3 (TLR3, CD283). Toll-like receptors (TLRs) are highly conserved from Drosophila to humans and share structural and functional similarities. TLRs constitute of a family of pattern recognition receptors (PRRs) that mediate cellular responses to a large variety of pathogens (viruses, bacteria, and parasites) by specific recognition of so-called ˜pathogen-associated molecular patterns. Activation of TLRs, a family of at least 11 different members that function either as homo- or heterodimers, leads to activation of NF degree Bdependent and IFN-regulatory factor-dependent signaling pathways. TLRs have a central role in innate immunity and are also required for the development of an adaptive immune response. TLRs are expressed by various cells of the immune system, such as macrophages and dendritic cells. TLRs are class I receptors, with a single alpha-helix that spans the cell membrane. They recognize and respond to molecules derived from bacterial, viral and fungal pathogens, such as lipopolysaccharide (LPS) from the outer membrane of Gram negative bacteria, peptidoglycan fragments from bacterial cell walls and single-stranded and double-stranded RNA from viruses.
Some forms of RNA and DNA from pathogens exhibit immutable features that distinguish them from nucleic acids of higher organisms. For example, dsRNA, is a common intermediate of viral replication and a potent indicator of infection. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA and its synthetic analog polyriboinosinic:polyribocytidylic acid (poly(I:C)). TLR3 is normally located in acidic endosomes where its luminal ectodomain (ECD) encounters dsRNA and induces type I interferon (IFN), inflammatory cytokine/chemokine production and dendritic cell (DC) maturation via the adaptor protein TICAM-1 (also called TRIF). Based on the different subcellular localization of cytosolic RNA receptors and TLR3, these receptors seem to play distinct roles in anti-viral immune responses.

References

1. Matsumoto, M et al; Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signalling. Biochem Biophys Res Commun 2002, 293: 1364 2. Oshiumi, H et al; TICAM-1, an adaptor molecule that participates in Toll-like receptor 3-mediated interferon-beta induction. Nat Immunol 2003, 4: 161
3. Matsumoto, M et al; Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 2003, 171: 3154
4. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184
5. Jorgenson, R et al; Human endometrial epithelial cells cyclically express Toll-like receptor 3 (TLR3) and exhibit TLR3-dependent responses to dsRNA. Human Immunology 2005; 66:469
6. Schreiner, B et al; Expression of Toll-like receptors by human muscle cells in vitro and in vivo: TLR3 is highly expressed in inflammatory and HIV myopathies, mediates IL-8 release, and up-regulation of NKG2D-ligands. Faseb 2006, 20:118

NCBI and Uniprot Product Information

• NCBI GI #: 86161330
• NCBI GeneID: 7098
• Molecular Weight: 72,937 Da
• NCBI Official Full Name: TLR3
• NCBI Official Synonym Full Names: toll-like receptor 3
• NCBI Official Symbol: TLR3
• NCBI Official Synonym Symbols: CD283; IIAE2
• NCBI Protein Information: toll-like receptor 3
• UniProt Protein Name: Toll-like receptor 3
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR3
• UniProt Entry Name: TLR3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses. Use of alternative polyadenylation sites to generate different length transcripts has been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR3: a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. Most abundantly expressed in placenta and pancreas. Restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection. Acts via MyD88 and TRAF6, leading to the activation of NF-kappaB and the production of type I interferon.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cell surface; membrane; integral to plasma membrane; lysosomal membrane; cytoplasm; endosome membrane

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; double-stranded RNA binding; receptor activity

Biological Process: negative regulation of osteoclast differentiation; I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of interferon-alpha biosynthetic process; positive regulation of NF-kappaB import into nucleus; toll-like receptor 3 signaling pathway; signal transduction; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; response to exogenous dsRNA; positive regulation of interferon-beta production; inflammatory response; defense response to virus; toll-like receptor 4 signaling pathway; MyD88-independent toll-like receptor signaling pathway; male gonad development; activation of NF-kappaB-inducing kinase; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor signaling pathway; positive regulation of chemokine production; hyperosmotic response; positive regulation of chemokine biosynthetic process; detection of virus; defense response to bacterium; positive regulation of interferon-beta biosynthetic process; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; I-kappaB phosphorylation; positive regulation of interferon-gamma biosynthetic process; microglial cell activation during immune response; positive regulation of inflammatory response

Disease: Human Immunodeficiency Virus Type 1, Susceptibility To