Mouse anti-Human TIP47 Monoclonal Antibody | anti-TIP47 antibody

TIP47 (M6PRBP1, Perilipin-3, 47kD Mannose 6-phosphate Receptor-binding Protein, 47kD MPR-binding Protein, Cargo Selection Protein TIP47, Mannose-6-phosphate Receptor-binding Protein 1, Placental Protein 17, PP17, PLIN3) (Biotin)

Cat. Num. AAA6144816

• Gene Names: PLIN3; PP17; TIP47; M6PRBP1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TIP47; Monoclonal Antibody; TIP47 (M6PRBP1; Perilipin-3; 47kD Mannose 6-phosphate Receptor-binding Protein; 47kD MPR-binding Protein; Cargo Selection Protein TIP47; Mannose-6-phosphate Receptor-binding Protein 1; Placental Protein 17; PP17; PLIN3) (Biotin); anti-TIP47 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2F7
• Specificity: Recognizes human M6PRBP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TIP47 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full-length recombinant protein corresponding to aa1-434 from M6PRBP1 (AAH01590) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHVKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQALSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (73.85kD).)

Western Blot (WB)

(Western Blot analysis of PLIN3 expression in transfected 293T cell line by 134464 Lane 1: PLIN3 transfected lysate (Predicted MW: 47kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of 134464 (3ug/ml) on formalin-fixed paraffin-embedded human tonsil.)

Immunoprecipitation (IP)

(Immunoprecipitation of PLIN3 transfected lysate using 134464 and Protein A Magnetic Bead, and immunoblotted with 134464.)

Testing Data

(Detection limit for recombinant GST tagged PLIN3 is 3ng/ml as a capture antibody.)

Product Categories/Family for anti-TIP47 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 33990761
• NCBI GeneID: 10226
• Molecular Weight: 47kDa
• NCBI Official Full Name: Homo sapiens mannose-6-phosphate receptor binding protein 1, mRNA
• NCBI Official Synonym Full Names: perilipin 3
• NCBI Official Symbol: PLIN3
• NCBI Official Synonym Symbols: PP17; TIP47; M6PRBP1
• NCBI Protein Information: perilipin-3

NCBI Description

Mannose 6-phophate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2009]