Mouse anti-Human Thyroid Stimulating Hormone, intact and beta Monoclonal Antibody
Thyroid Stimulating Hormone, intact and beta
Reactivity
Human
Applications
ELISA
Purity
Highly PurifiedPurified by DEAE chromatography.
Synonyms
Thyroid Stimulating Hormone; intact and beta; Monoclonal Antibody; Anti -Thyroid Stimulating Hormone; anti-Thyroid Stimulating Hormone; intact and beta antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
9L709
Specificity
Recognizes human TSH, intact and beta subunits.
Purity/Purification
Highly Purified
Purified by DEAE chromatography.
Purified by DEAE chromatography.
Form/Format
Supplied as a liquid in 10mM phosphate, pH 7.4, 150mM sodium chloride, 0.1% sodium azide.
Applicable Applications for anti-Thyroid Stimulating Hormone, intact and beta antibody
ELISA (EL/EIA)
Application Notes
Suitable for use in ELISA.
Immunogen
TSH from human pituitary gland.
Affinity Constant
2x10e10
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Thyroid Stimulating Hormone, intact and beta antibody
Thyroid-stimulating hormone (TSH or thyrotropin), produced in human pituitary glands, has a molecular mass of 25kD. An Important indicator of thyroid function, TSH is used to monitor thyroid associated diseases. Thyroid-stimulating hormone is a hormone synthesized and secreted by thyrotrope cells in the anterior pituitary gland which regulates the endocrine function of the thyroid gland. TSH stimulates the thyroid gland to secrete the hormones thyroxine (T4) and triiodothyronine (T3). TSH production is controlled by a Thyrotropin Releasing Hormone (TRH), which is manufactured in the hypothalamus and transported to the anterior pituitary gland, where it increases TSH production and release. Somatostatin is also produced by the hypothalamus, and has an opposite effect on the pituitary production of TSH, decreasing or inhibiting its release.
The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH. When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The alpha subunit is identical to that of human chorionic gonadotropin (HCG), luteinizing hormone (LH), follicle-stimulating hormone (FSH). The beta subunit is unique to TSH, and therefore determines its function.
TSH is a member of the cystine knot growth factor superfamily. It is a heterodimer of a 15kD unique subunit, TSH beta, with a 14kD alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Beta subunits of the four glycoprotein hormones share a 37-43% aa identity. Mature human TSH beta shares a 92%, 90%, 90%, 89%, 89%, 89%, and 88% aa identity with dog, rat, horse, mouse, cow, pig, and cat TSH b, respectively. Mature human CGa shares a 69%-73% aa identity with dog, rabbit, rat, mouse, cow, sheep, pig, cat and horse CGa. Each subunit forms a cystine knot structure with three disulfide bridges. A loop of the TSH beta subunit, termed a 'seat-belt', wraps around the CGa subunit to stabilize non-covalent association of the subunits, and also confers receptor selectivity.
Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but longer halflife. Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity. The hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland. TSH travels to thyroid TSHR to stimulate production of thyroxine (T4). In the tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production. Studies in mouse identify bone marrow as a secondary site of TSH production. In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling. Bone marrow cells that produce TSH may also circulate to the thyroid and appear to modulate thyroid hormone activity in times of immunological stress.
The level of Thyroid hormones (T3 and T4) in the blood have an additional effect on the pituitary release of TSH. When the levels of T3 and T4 are low, the production of TSH is increased, and conversely, when levels of T3 and T4 are high, then TSH production is decreased. This effect creates a regulatory negative feedback loop. TSH is a glycoprotein and consists of two subunits, the alpha and the beta subunit. The alpha subunit is identical to that of human chorionic gonadotropin (HCG), luteinizing hormone (LH), follicle-stimulating hormone (FSH). The beta subunit is unique to TSH, and therefore determines its function.
TSH is a member of the cystine knot growth factor superfamily. It is a heterodimer of a 15kD unique subunit, TSH beta, with a 14kD alpha subunit, CGa (common glycoprotein hormone alpha) that is shared with lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Beta subunits of the four glycoprotein hormones share a 37-43% aa identity. Mature human TSH beta shares a 92%, 90%, 90%, 89%, 89%, 89%, and 88% aa identity with dog, rat, horse, mouse, cow, pig, and cat TSH b, respectively. Mature human CGa shares a 69%-73% aa identity with dog, rabbit, rat, mouse, cow, sheep, pig, cat and horse CGa. Each subunit forms a cystine knot structure with three disulfide bridges. A loop of the TSH beta subunit, termed a 'seat-belt', wraps around the CGa subunit to stabilize non-covalent association of the subunits, and also confers receptor selectivity.
Structure and charge of the three N-linked carbohydrate chains influence activity; the most complex forms have lower activity but longer halflife. Bovine and porcine TSH bind human TSH receptors (TSHR) with high affinity. The hypothalamic peptide TRH stimulates production and secretion of TSH by thyrotrophs (basophile cells) in the anterior pituitary gland. TSH travels to thyroid TSHR to stimulate production of thyroxine (T4). In the tissues, T4 is converted to the active form of thyroid hormone, triiodothyronine (T3), which completes a feedback loop by inhibiting TSH production. Studies in mouse identify bone marrow as a secondary site of TSH production. In bone, TSH signaling through TSHR on osteoblast and osteoclast precursors negatively regulates skeletal remodeling. Bone marrow cells that produce TSH may also circulate to the thyroid and appear to modulate thyroid hormone activity in times of immunological stress.
Product Categories/Family for anti-Thyroid Stimulating Hormone, intact and beta antibody
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Product Notes
The Thyroid Stimulating Hormone, intact and beta (Catalog #AAA607893) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Thyroid Stimulating Hormone, intact and beta reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thyroid Stimulating Hormone, intact and beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in ELISA. Researchers should empirically determine the suitability of the Thyroid Stimulating Hormone, intact and beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyroid Stimulating Hormone, intact and beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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