anti-Mouse TCR alpha beta Monoclonal Antibody

Hamster Anti-Mouse TCRb-FITC

Cat. Num. AAA673506

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: TCR alpha beta; Monoclonal Antibody; Hamster Anti-Mouse TCRb-FITC; Hamster Anti-Mouse TCRb - FITC; Hamster Anti-Mouse TCR alpha beta; anti-TCR alpha beta antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG
• Clone Number: H57-597
• Specificity: Common epitope of the beta chain of the mouse alpha beta T cell receptor (TCR) complex; The alpha beta TCR is expressed on T lymphocytes of all mouse strains tested.2 The H57-597 monoclonal antibody does not react with gamma delta TCR-bearing cells.2 Plate-bound or soluble H57-597 activates alpha beta TCRbearing T cells.2 In vitro and in vivo, H57-597 can induce immature thymocytes to undergo apoptosis.3,4 In vivo administration of H57-597 has been shown to deplete alpha beta TCR-bearing cells to near completion and prevent graft rejection.5
• Form/Format: Fluorescein (FITC) Conjugate

• Applicable Applications for anti-TCR alpha beta antibody: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): In vitro stimulation of alpha beta TCR-expressing T cells; In vitro and in vivo depletion of alpha beta TCR-bearing cells; ; Flow Cytometry Suggested Dilution: <= 2 ug/10^6 cells
• Immunogen: TCR affinity purified from mouse T-cell hybridoma DO-11.10
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative flow data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; The Alexa Fluor 488 (AF488) and Alexa Fluor 647 (AF647) conjugates are supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Gascoigne, N. R. J. 1990. J. Biol. Chem. 265:9296.
2. Kubo, R. T., W. Born, J. W. Kappler, P. Marrack, and M. Pigeon. 1989. J. Immunol. 142:2736.
3. Finkel, T. H., J. C. Cambier, R. T. Kubo, W. K. Born, P. Marrack, and J. W. Kappler. 1989. Cell 58:1047.
4. Shi, Y. F., R. P. Bissonnette, N. Parfrey, M. Szalay, R. T. Kubo, and D. R. Green. 1991. J. Immunol. 146:3340.
5. Murosaki, S., Y. Yoshikai, A. Ishida, T. Nakamura, G. Matsuzaki, H. Takimoto, H. Yuuki, and K. Nomoto. 1991. Int. Immunol. 3:1005.
6. Finkel, T. H., P. Marrack, J. W. Kappler, R. T. Kubo, and J. C. Cambier. 1989. J. Immunol. 142:3006.
7. Iwata, M., K. Katamura, R. T. Kubo, and K. Ishizaka. 1989. J. Immunol. 143:3909.
8. Iwata, M., K. Katamura, R. T. Kubo, H. M. Grey, and K. Ishizaka. 1989. J. Immunol. 143:3917.
9. Byrne, J. A., J. L. Butler, E. L. Reinherz, and M. D. Cooper. 1989. Int. Immunol. 1:29.