Western Blot (WB)
(TCF4 monoclonal antibody, Western Blot analysis of TCF4 expression in K-562.)
Mouse anti-Human TCF4 Monoclonal Antibody | anti-TCF4 antibody
TCF4 (Transcription Factor 4, TCF-4, Class B Basic Helix-loop-helix Protein 19, bHLHb19, Immunoglobulin Transcription Factor 2, ITF-2, SEF-2, SL3-3 Enhancer Factor 2, BHLHB19, ITF2, SEF2, MGC149723, MGC149724) (AP)
Gene Names
TCF4; E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TCF4; Monoclonal Antibody; TCF4 (Transcription Factor 4; TCF-4; Class B Basic Helix-loop-helix Protein 19; bHLHb19; Immunoglobulin Transcription Factor 2; ITF-2; SEF-2; SL3-3 Enhancer Factor 2; BHLHB19; ITF2; SEF2; MGC149723; MGC149724) (AP); anti-TCF4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1G4
Specificity
Recognizes human TCF4.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-TCF4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-365 from human TCF4 with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MHHQQRMAALGTDKELSDLLDFSAMFSPPVSSGKNGPTSLASGHFTGSNVEDRSSSGSWGNGGHPSPSRNYGDGTPYDHTTSRDLGSHDNLSPPFVNSRIQSKTERGSYSSYGRESNLQGCHQQSLLGGDMDMGNPGTLSPTKPGSQYYQYSSNNPRRRPLHSSAMEVQTKKVRKVPPGLPSSVYAPSASTADYNRDSPGYPSSKPATSTFPSSFFMQDGHHSSDPWSSSSGMNQPGYAGMLGNSSHIPQSSSYCSLHPHERLSYPSHSSADINSSLPPMSTFHRSGTNHYSTSSCTPPANGTDSIMANRGSGAAGSSQTGDALGKALASIYSPDHTNNSFSSNPSTPVGSPPSLSAGTAVWSRN
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(TCF4 monoclonal antibody, Western Blot analysis of TCF4 expression in K-562.)
Western Blot (WB)
(TCF4 monoclonal antibody, Western Blot analysis of TCF4 expression in K-562.)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to TCF4 on HeLa cell. [antibody concentration 10ug/ml])
Testing Data
(Detection limit for recombinant GST tagged TCF4 is ~1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged TCF4 is ~1ng/ml as a capture antibody.)
Product Categories/Family for anti-TCF4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
48,098 Da
NCBI Official Full Name
Homo sapiens transcription factor 4, mRNA
NCBI Official Synonym Full Names
transcription factor 4
NCBI Official Symbol
TCF4
NCBI Official Synonym Symbols
E2-2; ITF2; PTHS; SEF2; FECD3; ITF-2; SEF-2; TCF-4; SEF2-1; SEF2-1A; SEF2-1B; SEF2-1D; bHLHb19
NCBI Protein Information
transcription factor 4
Protein Family
NCBI Description
This gene encodes transcription factor 4, a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. In addition, an intronic CTG repeat normally numbering 10-37 repeat units can expand to >50 repeat units and cause Fuchs endothelial corneal dystrophy. Multiple alternatively spliced transcript variants that encode different proteins have been described. [provided by RefSeq, Jul 2016]
Research Articles on TCF4
Similar Products
Product Notes
The TCF4 (Catalog #AAA6134146) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TCF4 (Transcription Factor 4, TCF-4, Class B Basic Helix-loop-helix Protein 19, bHLHb19, Immunoglobulin Transcription Factor 2, ITF-2, SEF-2, SL3-3 Enhancer Factor 2, BHLHB19, ITF2, SEF2, MGC149723, MGC149724) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCF4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TCF4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCF4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
