Mouse anti-Human, Mouse TBP Monoclonal Antibody | anti-TBP antibody

TBP Antibody

Cat. Num. AAA9232190

• Gene Names: TBP; HDL4; GTF2D; SCA17; TFIID; GTF2D1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Synonyms: TBP; Monoclonal Antibody; TBP Antibody; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit; GTF2D1; TF2D; TFIID; anti-TBP antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Purified His-tagged TBP protein was used to produced this monoclonal antibody.
• Sequence Length: 319

• Applicable Applications for anti-TBP antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: IHC: 1:25; WB: 1:2000
• Antigen Source: Human
• Antigen Region: 1-288
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-TBP Antibody at 1:3000 dilutionLane 1: Hela whole cell lysateLane 2: A431 whole cell lysateLane 3: A549 whole cell lysateLane 4: HepG2 whole cell lysateLane 5: NIH/3T3 whole cell lysateLane 6: C2C12 whole cell lysateLane 7: PC-12 whole cell lysateLane 8: C6 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Staining TBP in human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Staining TBP in Rat brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Staining TBP in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Staining TBP in Monkey. brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Western Blot (WB)

(All lanes : Anti-TBP Antibody at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: 293T/17 whole cell lysateLane 3: NIH/3T3 whole cell lysateLane 4: Hela whole cell lysateLane 5: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-TBP antibody

General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Product Categories/Family for anti-TBP antibody

Neuroscience

References

Hoffmann A., et al. Nature 346:387-390(1990). Peterson M.G., et al. Science 248:1625-1630(1990). Kao C.C., et al. Science 248:1646-1650(1990). Ebert L., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Mungall A.J., et al. Nature 425:805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 285026520
• NCBI GeneID: 6908
• NCBI Accession #: NP_001165556.1; NP_003185.1
• NCBI GenBank Nucleotide #: NP_001165556.1
• UniProt Accession #: P20226
• Molecular Weight: 37698
• NCBI Official Full Name: TATA-box-binding protein isoform 2
• NCBI Official Synonym Full Names: TATA-box binding protein
• NCBI Official Symbol: TBP
• NCBI Official Synonym Symbols: HDL4; GTF2D; SCA17; TFIID; GTF2D1
• NCBI Protein Information: TATA-box-binding protein
• UniProt Protein Name: TATA-box-binding protein
• Protein Family: TBP-like protein
• UniProt Gene Name: TBP
• UniProt Synonym Gene Names: GTF2D1; TF2D; TFIID
• UniProt Entry Name: TBP_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 25-42, and expansion of the number of repeats to 45-66 increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2016]

Uniprot Description

TBP: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1. Interacts with CITED2. Interacts with ATF7IP. Interacts with HIV-1 Tat. Widely expressed, with levels highest in the testis and ovary. Belongs to the TBP family.

Protein type: Transcription initiation complex; DNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: nucleoplasm; transcription factor TFIID complex; male pronucleus; cytoplasm; female pronucleus; transcription factor TFIIA complex

Molecular Function: protein binding; transcription factor binding; transcription factor activity

Biological Process: chromatin silencing at rDNA; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; positive regulation of transcription, DNA-dependent; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase II promoter; gene expression; spermatogenesis; transcription initiation from RNA polymerase I promoter

Disease: Parkinson Disease, Late-onset; Spinocerebellar Ataxia 17