Mouse anti-Human Tau, Nitrated Monoclonal Antibody | anti-Tpi1 antibody

Tau, Nitrated (Tyr29) (microtubule-associated protein tau, DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, Microtubule-associated protein tau, MSTD, MTBT1, MTBT2, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, PPND, tau, TAU)

Cat. Num. AAA606770

• Gene Names: Tpi1; Tpi; Tpi-1; AI255506
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Tau; Nitrated; Monoclonal Antibody; Nitrated (Tyr29) (microtubule-associated protein tau; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; Microtubule-associated protein tau; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; tau; TAU); Anti -Tau; anti-Tpi1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 9H282
• Specificity: Recognizes nitrated human Tau (Tyr29).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-Tpi1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.
• Immunogen: KLH conjugated synthetic linear peptide corresponding to the amino acids surrounding and including nitrated Tyr29 of human Tau.
• Positive Control: Tau (Nitrated Tyr29)/Tau (Nitrated Tyr18) lysate. Tau (Nitrated Tyr18) lysate is a; negative control.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Tpi1 antibody

Microtubule Associated Proteins, or MAPs, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, Iand II. Type II MAP includes MAP2, MAP4, and tau and is found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Product Categories/Family for anti-Tpi1 antibody

Antibodies; Abs to Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 226958349
• NCBI GeneID: 21991
• NCBI Accession #: NP_033441.2
• NCBI GenBank Nucleotide #: NM_009415.2
• UniProt Accession #: P17751; Q3TJH2; Q3UC04; Q3UKG9; Q64513; Q9CVF9; Q9CWE7
• Molecular Weight: 32,192 Da
• NCBI Official Full Name: triosephosphate isomerase
• NCBI Official Synonym Full Names: triosephosphate isomerase 1
• NCBI Official Symbol: Tpi1
• NCBI Official Synonym Symbols: Tpi; Tpi-1; AI255506
• NCBI Protein Information: triosephosphate isomerase; TIM; triose-phosphate isomerase
• UniProt Protein Name: Triosephosphate isomerase
• UniProt Gene Name: Tpi1
• UniProt Synonym Gene Names: Tpi; TIM
• UniProt Entry Name: TPIS_MOUSE

Uniprot Description

TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Carbohydrate Metabolism - inositol phosphate; Isomerase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; EC 5.3.1.1

Cellular Component: extracellular space; nucleus

Molecular Function: isomerase activity; catalytic activity; triose-phosphate isomerase activity

Biological Process: pentose-phosphate shunt; embryonic development; glycolysis; metabolic process; multicellular organismal development; glyceraldehyde-3-phosphate metabolic process; glucose metabolic process; gluconeogenesis