Mouse TAF15 Monoclonal Antibody | anti-TAF15 antibody

TAF15 Antibody

Cat. Num. AAA415286

• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: TAF15; Monoclonal Antibody; TAF15 Antibody; anti-TAF15 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-TAF15 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: 1:100-1:500 for immunofluorescence. For western blots try at 1:500.
• Immunogen: TAF15 is a member of a family of 3 closely related mammalian RNA binding proteins, the other members being FUS/TLS and EWSR1. TDP43 is a more distant relative of these three. The protein is also known as RBP56, for RNA binding protein 56kDa (1). The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for this protein is TAF15. As with the other members of this family, TAF15 contains one copy of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. Like TDP43, Fus/TLS and EWS, TAF15 is widely expressed in tissues and is localized primarily in the nucleus of cells (2). It was recently shown that point mutations in TAF15 may be associated with some familial forms of Lou Gehrig's disease, also known as amyotrophic lateral sclerosis or ALS (3). Fus/TLS and TDP43 protein mutations and aggregation have been implicated in various kinds of neurological disease including ALS (4). MCA-4D71 was generated against full length recombinant human TAF15 expressed in and purified from E. coli. For our antibody to TDP43, see MCA-3H8. The HGNC name for this protein is TAF15.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

References

1. Morohoshi F, Arai K, Takahashi EI, Tanigami A, Ohki M. Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins. Genomics 1996 38:51-7 (1996).2. Andersson MK, Ståhlberg A, Arvidsson Y, Olofsson A, Semb H, Stenman G, Nilsson O, Aman P. The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response. BMC Cell Biol. 11:9:37 (2008).3. Ticozzi N, Vance C, Leclerc AL, Keagle P, Glass JD, McKenna-Yasek D, Sapp PC, Silani V, Bosco DA, Shaw CE, Brown RH Jr, Landers JE. Mutational analysis reveals the FUS homolog TAF15 as a candidate gene for familial amyotrophic lateral sclerosis. Am J Med Genet B Neuropsychiatr Genet. [Epub ahead of print] (2011).4. Da Cruz S, Cleveland DW. Understanding the role of TDP-43 and FUS/TLS in ALS and beyond. Curr Opin Neurobiol. [Epub ahead of print] (2011).