Rat T-complex Protein 1 epsilon Monoclonal Antibody | anti-CCT5 antibody

T-complex Protein 1 epsilon (T-complex Protein 1 epsilon Subunit, TCP1 epsilon, TCP1-epsilon, TCP-1 epsilon, Chaperonin Containing TCP1 epsilon, Chaperonin-containing TCP-1 epsilon, CCT epsilon, CCT-epsilon, CCTE, Chaperonin Containing TCP1 Subunit 5, Cha

Cat. Num. AAA604604

• Gene Names: CCT5; CCTE; KIAA0098; CCT-epsilon; TCP-1-epsilon
• Applications: Western Blot, Immunoprecipitation, Gel Super Shift Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: T-complex Protein 1 epsilon; Monoclonal Antibody; T-complex Protein 1 epsilon (T-complex Protein 1 epsilon Subunit; TCP1 epsilon; TCP1-epsilon; TCP-1 epsilon; Chaperonin Containing TCP1 epsilon; Chaperonin-containing TCP-1 epsilon; CCT epsilon; CCT-epsilon; CCTE; Chaperonin Containing TCP1 Subunit 5; Cha; Anti -T-complex Protein 1 epsilon (T-complex Protein 1 epsilon Subunit; anti-CCT5 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 10B490
• Specificity: Recognizes the epsilon polypeptide of the human CCT chaperonin molecule complex at ~60kD. Species Crossreactivity: Mouse, Rabbit, Rat, Bovine
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-CCT5 antibody: Western Blot (WB), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA)
• Application Notes: Suitable for use in Gel Super Shift Assay, Immunoprecipitation, and Western Blot.; Dilution: Western Blot: 1:250-1:500
• Immunogen: CCT Epsilon.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CCT5 antibody

The intact CCT complex is composed of eight polypeptides in a double-ring structure. CCT is important within cells in aiding the folding of proteins including actin, tubulin and the VHL tumor suppressor protein.

Product Categories/Family for anti-CCT5 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 24307939
• NCBI GeneID: 22948
• NCBI Accession #: NP_036205.1
• NCBI GenBank Nucleotide #: NM_012073.3
• UniProt Accession #: P48643; A8JZY8; Q96GI1; Q9BU08; Q9HB74
• Molecular Weight: 59,671 Da
• NCBI Official Full Name: T-complex protein 1 subunit epsilon
• NCBI Official Synonym Full Names: chaperonin containing TCP1, subunit 5 (epsilon)
• NCBI Official Symbol: CCT5
• NCBI Official Synonym Symbols: CCTE; KIAA0098; CCT-epsilon; TCP-1-epsilon
• NCBI Protein Information: T-complex protein 1 subunit epsilon; OTTHUMP00000161671; OTTHUMP00000220727; OTTHUMP00000220728; OTTHUMP00000220953; OTTHUMP00000221653; T-complex protein 1, epsilon subunit
• UniProt Protein Name: T-complex protein 1 subunit epsilon
• Protein Family: T-complex protein
• UniProt Gene Name: CCT5
• UniProt Synonym Gene Names: CCTE; KIAA0098
• UniProt Entry Name: TCPE_HUMAN

NCBI Description

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. In addition, three pseudogenes of this gene have been identified. [provided by RefSeq]

Uniprot Description

CCT-epsilon: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Defects in CCT5 are the cause of hereditary sensory neuropathy autosomal recessive with spastic paraplegia (HSNSP). The disease is characterized by spastic paraplegia and progressive distal sensory neuropathy leading to mutilating ulcerations of the upper and lower limbs. Belongs to the TCP-1 chaperonin family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: chaperonin-containing T-complex; microtubule; centrosome; cytoplasm; nucleolus; cytosol

Molecular Function: protein binding; unfolded protein binding; beta-tubulin binding; G-protein beta-subunit binding; ATP binding

Biological Process: 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida; response to virus

Disease: Neuropathy, Hereditary Sensory, With Spastic Paraplegia, Autosomal Recessive