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Western Blot (WB)

Mouse anti-Mouse, Rat SYP/Synaptophysin Monoclonal Antibody | anti-SYP antibody

Anti-SYP/Synaptophysin Antibody (Monoclonal, 3G12)

Gene Names
SYP; MRX96; MRXSYP
Reactivity
Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
SYP/Synaptophysin; Monoclonal Antibody; Anti-SYP/Synaptophysin Antibody (Monoclonal; 3G12); MRXSYP; Major synaptic vesicle protein p38; anti-SYP antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3G12
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
313
Applicable Applications for anti-SYP antibody
Western blot (WB)
Application Notes
WB: 0.1-0.5ug/ml (Mouse, Rat)
Tested Species: In-house tested species with positive results.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin(1-26aa MLLLADMDVVNQLVAGGQFRVVKEPL), identical to the related mouse and rat sequences.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

Western Blot (WB)
Related Product Information for anti-SYP antibody
Description: Mouse IgG monoclonal antibody for SYP/Synaptophysin detection. Tested with WB in Mouse; Rat.

Background: SYP(Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).
References
1. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996). "Comparative evaluation of synaptophysin-based methods for quantification of synapses". J. Neurocytol.25 (12): 821-8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
synaptophysin
NCBI Official Synonym Full Names
synaptophysin
NCBI Official Symbol
SYP
NCBI Official Synonym Symbols
MRX96; MRXSYP
NCBI Protein Information
synaptophysin
UniProt Protein Name
Synaptophysin
Protein Family
UniProt Gene Name
SYP
UniProt Entry Name
SYPH_HUMAN

NCBI Description

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: presynaptic membrane; synaptic vesicle; neuron projection; protein complex; synaptic vesicle membrane; presynaptic active zone; excitatory synapse; cell junction; integral to synaptic vesicle membrane

Molecular Function: identical protein binding; protein self-association; transporter activity; protein complex binding; cholesterol binding; syntaxin-1 binding; SH2 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis; endocytosis; regulation of short-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity

Disease: Mental Retardation, X-linked 96

Research Articles on SYP

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Product Notes

The SYP syp (Catalog #AAA1753247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-SYP/Synaptophysin Antibody (Monoclonal, 3G12) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SYP/Synaptophysin can be used in a range of immunoassay formats including, but not limited to, Western blot (WB). WB: 0.1-0.5ug/ml (Mouse, Rat) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the SYP syp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYP/Synaptophysin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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