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Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml])
Mouse SURB7 Monoclonal Antibody | anti-SURB7 antibody
SURB7 (Mediator Complex Subunit 21, SRB7, SURB7) (Biotin)
Gene Names
MED21; SRB7; SURB7; hSrb7
Applications
Immunofluorescence, Western Blot
Purity
Purified
Synonyms
SURB7; Monoclonal Antibody; SURB7 (Mediator Complex Subunit 21; SRB7; SURB7) (Biotin); Mediator Complex Subunit 21; anti-SURB7 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4B5
Specificity
Recognizes SURB7.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
144
Applicable Applications for anti-SURB7 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
SURB7 (AAH08380, 1aa-144aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml])
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to SURB7 on HepG2 cell. [antibody concentration 35 ug/ml])
Testing Data
(Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged SURB7 is approximately 0.03ng/ml as a capture antibody.)
Western Blot (WB)
(Western Blot analysis of MED21 expression in transfected 293T cell line by SURB7 monoclonal antibody (M05), clone 4B5.Lane 1: MED21 transfected lysate (Predicted MW: 15.6 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of MED21 expression in transfected 293T cell line by SURB7 monoclonal antibody (M05), clone 4B5.Lane 1: MED21 transfected lysate (Predicted MW: 15.6 KDa).Lane 2: Non-transfected lysate.)
Related Product Information for anti-SURB7 antibody
Mouse monoclonal antibody raised against a full length recombinant SURB7.
Product Categories/Family for anti-SURB7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Mediator complex subunit 21
NCBI Official Synonym Full Names
mediator complex subunit 21
NCBI Official Symbol
MED21
NCBI Official Synonym Symbols
SRB7; SURB7; hSrb7
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 21
NCBI Description
This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]
Research Articles on SURB7
Similar Products
Product Notes
The SURB7 (Catalog #AAA6172326) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's SURB7 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SURB7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SURB7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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