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Western Blot (WB) (Western Blot detection against Immunogen (82.65kD).)

Mouse anti-Human STK33 Monoclonal Antibody | anti-STK33 antibody

STK33 (Serine/Threonine Kinase 33) (AP)

Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
STK33; Monoclonal Antibody; STK33 (Serine/Threonine Kinase 33) (AP); anti-STK33 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6G1
Specificity
Recognizes human STK33.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-STK33 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-515 from human STK33 (AAH31231) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEGKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPETNYTSDEEEEKQSTTYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (82.65kD).)

Western Blot (WB) (Western Blot detection against Immunogen (82.65kD).)

Western Blot (WB)

(Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody. Lane 1: STK33 transfected lysate (57.9kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody. Lane 1: STK33 transfected lysate (57.9kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 25ug/ml].)

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 25ug/ml].)

Immunoprecipitation (IP)

(Immunoprecipitation of STK33 transfected lysate using STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 rabbit polyclonal antibody.)

Immunoprecipitation (IP) (Immunoprecipitation of STK33 transfected lysate using STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 rabbit polyclonal antibody.)

Western Blot (WB)

(Western blot analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB)

(STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)

Western Blot (WB) (STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)
Product Categories/Family for anti-STK33 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
50,176 Da
NCBI Official Full Name
Homo sapiens serine/threonine kinase 33, mRNA
NCBI Official Synonym Full Names
serine/threonine kinase 33
NCBI Official Symbol
STK33
NCBI Protein Information
serine/threonine-protein kinase 33

Research Articles on STK33

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Product Notes

The STK33 (Catalog #AAA6134037) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STK33 (Serine/Threonine Kinase 33) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STK33 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the STK33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STK33, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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