Mouse anti-Human, Mouse STAT6 Monoclonal Antibody | anti-STAT6 antibody

STAT6 phosphorylated (Y641) (STAT6B, STAT6C, D12S1644, IL-4-STAT, interleukin 4 induced STAT, Signal Transducer and Activator of Transcription 6) (BSA & Azide Free)

Cat. Num. AAA6120717

• Gene Names: STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
• Reactivity: Human, Mouse
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: STAT6; Monoclonal Antibody; STAT6 phosphorylated (Y641) (STAT6B; STAT6C; D12S1644; IL-4-STAT; interleukin 4 induced STAT; Signal Transducer and Activator of Transcription 6) (BSA & Azide Free); anti-STAT6 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4H253
• Specificity: Recognizes human STAT 6 (Signal Transducers and Activators of Transcription 6), phosphorylated (Y641). Species Cross Reactivity: Mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS. No stabilizing proteins added. No preservative added. Also available with BSA and Azide.
• Concentration: ~0.5mg/ml (varies by lot)

• Applicable Applications for anti-STAT6 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1-2ug/ml; WB: 1-2ug/ml
• Immunogen: Synthetic peptide corresponding to amino acids 631-645 containing a phospho-tyrosine residue of human Stat6.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of phosphorylated Stat6 in NIH 3T3 cells using S7972-26C. Lane A. Untreated cell lysate. Lane B and C. Anisomycin or EGF treated cell lysates, respectively.)

Related Product Information for anti-STAT6 antibody

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Stat6 is rapidly tyrosine phosphorylated following stimulation of appropriate cell lines with IL-3, IL-4, epidermal growth factor (EGF), but is not detectably phosphorylated following stimulation with IL-2, IL-12, or erythropoietin.

Product Categories/Family for anti-STAT6 antibody

Antibodies; Transcription Factors; STAT

References

1. Leonard WJ, O'Shea JJ. Jaks and STATs: biological implications. Annu Rev Immunol 16:293-322 (1998). 2. Schindler C, Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem 64:621-51 (1995). 3. Bluyssen HA and Levy DE. J Biol Chem 272: 4600-4605 (1997). 4. Darnell JE Jr. STATs and gene regulation. Science 277:1630-1635 (1997). 5. Quelle FW, Shimoda K, Thierfelder et al. Mol Cell Biol 15(6): 3336-43. (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 23397678
• NCBI GeneID: 6778
• NCBI Accession #: NP_003144
• NCBI GenBank Nucleotide #: NM_003153.4
• Molecular Weight: 81,748 Da
• NCBI Official Full Name: signal transducer and activator of transcription 6 isoform 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 6, interleukin-4 induced
• NCBI Official Symbol: STAT6
• NCBI Official Synonym Symbols: STAT6B; STAT6C; D12S1644; IL-4-STAT
• NCBI Protein Information: signal transducer and activator of transcription 6
• Protein Family: Signal transducer and transcription activator

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein plays a central role in exerting IL4 mediated biological responses. It is found to induce the expression of BCL2L1/BCL-X(L), which is responsible for the anti-apoptotic activity of IL4. Knockout studies in mice suggested the roles of this gene in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]