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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant STAT5A protein (Right) or empty vector (Left) detected with STAT5A antibody)

Mouse STAT5A Monoclonal Antibody | anti-STAT5A antibody

STAT5A antibody

Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
STAT5A antibody was purified by affinity chromatography.
Synonyms
STAT5A; Monoclonal Antibody; STAT5A antibody; Monoclonal STAT5A; Anti-STAT5A; Signal transducer and activator of transcription 5A; MGF; STAT5; STATA 5; STATA-5; anti-STAT5A antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6D6
Purity/Purification
STAT5A antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
51
Applicable Applications for anti-STAT5A antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:150
WB: 1:2000
Biological Significance
STAT5A is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein isactivated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with aTEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis.
Cross-Reactivity
Human
Immunogen
STAT5A antibody was raised in mouse using a full length recombinant protein of human STAT5A (NP_003143) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant STAT5A protein (Right) or empty vector (Left) detected with STAT5A antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant STAT5A protein (Right) or empty vector (Left) detected with STAT5A antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of STAT5A protein in paraffin embedded Human lymphoma tissue using STAT5A antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of STAT5A protein in paraffin embedded Human lymphoma tissue using STAT5A antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant STAT5A protein using STAT5A antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant STAT5A protein using STAT5A antibody)
Related Product Information for anti-STAT5A antibody
Mouse monoclonal STAT5A antibody
Product Categories/Family for anti-STAT5A antibody

NCBI and Uniprot Product Information

NCBI GI #
Molecular Weight
6,170 Da
NCBI Official Full Name
STAT5A, partial
UniProt Protein Name
STAT5A
UniProt Gene Name
STAT5A
UniProt Entry Name
A0A023VXS8_BUBBU

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Product Notes

The STAT5A stat5a (Catalog #AAA838649) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's STAT5A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:150 WB: 1:2000. Researchers should empirically determine the suitability of the STAT5A stat5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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