Mouse anti-Human STAT 5b Monoclonal Antibody | anti-STAT5B antibody

STAT 5b, phosphorylated (Ser133) (Signal Transducer and Activator of Transcription 5b)

Cat. Num. AAA633318

• Gene Names: STAT5B; STAT5
• Reactivity: Human
• Applications: ELISA
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: STAT 5b; Monoclonal Antibody; phosphorylated (Ser133) (Signal Transducer and Activator of Transcription 5b); Anti -STAT 5b; anti-STAT5B antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 9A131
• Specificity: Reactive to the immunogen human STAT5b, phosphorylated (pS133) (Signal Transducers and Activators of Transcription 5b), peptide-BSA conjugating and peptide in ELISA.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a solution of 0.01M PBS, pH7.0. Reconstitution: Double distillated water is recommended and to adjust the final concentration to 1mg/ml.

• Applicable Applications for anti-STAT5B antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA., , Recommended Dilution:, Indirect ELISA: 1ug/ml of peptide/or peptide conjugate in pH 9.5 carbonate buffer, 100ul/well, Optimal dilutions to be determined by the researcher.
• Immunogen: Peptide of a phosphorylated fragment of CREB [p-S133], conjugated with KLH.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 24 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-STAT5B antibody

STAT (signal transducers and activators of transcription) can be selectively phosphorylated on tyrosine and serine residues. The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, thought to be essential for their selective activity. STAT5 can be activated by a variety of different agents including prolactin, IL-2, IL-3, IL-7, IL-5, growth hormone and erythropoietin. The highly homologous STAT 5a and STAT 5b proteins are essential mediators of prolactin and growth hormone effects and have been implicated in physiological functions including cytokine control of apoptosis, growth, reproduction, lactation, immune function, differentiation and somatic growth.

Product Categories/Family for anti-STAT5B antibody

Antibodies; Abs to Transcription Factors; STAT

NCBI and Uniprot Product Information

• NCBI GI #: 21618344
• NCBI GeneID: 6777
• NCBI Accession #: NP_036580.2
• NCBI GenBank Nucleotide #: NM_012448.3
• UniProt Accession #: P51692; Q8WWS8; Q59H39; Q8WW55
• Molecular Weight: 89,866 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5B
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5B
• NCBI Official Symbol: STAT5B
• NCBI Official Synonym Symbols: STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5B; OTTHUMP00000198374; transcription factor STAT5B
• UniProt Protein Name: Signal transducer and activator of transcription 5B
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5B
• UniProt Entry Name: STA5B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq]

Uniprot Description

STAT5B: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases triggered by cytokines including IL2, IL3, GM-CSF, and various growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein dimerization activity; signal transducer activity; protein binding; double-stranded DNA binding; chromatin binding; protein phosphatase binding; transcription factor activity; glucocorticoid receptor binding

Biological Process: positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; response to lipopolysaccharide; positive regulation of multicellular organism growth; female pregnancy; 2-oxoglutarate metabolic process; sequestering of lipid; allantoin metabolic process; natural killer cell differentiation; regulation of steroid metabolic process; acute-phase response; T cell homeostasis; Peyer's patch development; isoleucine metabolic process; positive regulation of natural killer cell proliferation; valine metabolic process; development of secondary female sexual characteristics; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; response to ethanol; regulation of epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; negative regulation of apoptosis; lactation; transcription from RNA polymerase II promoter; succinate metabolic process; oxaloacetate metabolic process; positive regulation of smooth muscle cell proliferation; progesterone metabolic process; positive regulation of mitotic cell cycle; fatty acid metabolic process; positive regulation of activated T cell proliferation; response to estradiol stimulus; positive regulation of natural killer cell differentiation; luteinization; development of secondary male sexual characteristics; creatinine metabolic process; positive regulation of gamma-delta T cell differentiation; negative regulation of erythrocyte differentiation; citrate metabolic process; regulation of multicellular organism growth; positive regulation of cell motility; positive regulation of natural killer cell mediated cytotoxicity; liver development; creatine metabolic process; cellular response to hormone stimulus; response to hypoxia; positive regulation of B cell differentiation; positive regulation of inflammatory response

Disease: Growth Hormone Insensitivity With Immunodeficiency