Mouse anti-Human SSU72 Monoclonal Antibody | anti-SSU72 antibody

SSU72 antibody

Cat. Num. AAA206585

• Gene Names: SSU72; HSPC182; PNAS-120
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: By protein-A affinity chromatography
• Synonyms: SSU72; Monoclonal Antibody; SSU72 antibody; SSu72 RNA polymerase II CTD phosphatase homolog; HSPC182; PNAS-120; anti-SSU72 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: AT66D10
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 194

• Applicable Applications for anti-SSU72 antibody: ELISA (EIA), Western Blot (WB)
• Antigen Species: Human
• Immunogen: Recombinant human SSU72 (1-194aa) purified from E. coli
• Clone Note: Anti-human SSU72 mAb, clone AT66D10, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SSU72 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SSU72 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: K562 cell lysate Lane 3.: 293T cell lysate Lane 4.: HepG2 cell lysate Lane 5.: A549 cell lysate Lane 6.: MCF-7 cell lysate Lane 7.: SK-OV-3 cell lysate Lane 8.: LnCap cell lysate Lane 9.: HeLa cell lysate)

Related Product Information for anti-SSU72 antibody

SSU72 RNA polymerase II CTD phosphatase homolog, also known as SSU72, is a highly conserved homologue of yeast Ssu72, a CTD phosphatase and a component of the polyadenylation/ termination machinery. Existing as multiple alternatively spliced isoforms, SSU72 interacts with TFIIB, Rb and DNAM-1 and functions to catalyze the dephosphorylation of target proteins, possibly playing a role in RNA processing and termination via dephosphorylation of Pol II.

Product Categories/Family for anti-SSU72 antibody

Epigenomics

References

Dichtl B., et al. (2002) Mol Cell. 10: 1139-1150.; Krishnamurthy S., et al. (2004) Mol Cell. 14: 387-394.

NCBI and Uniprot Product Information

• NCBI GI #: 7661832
• NCBI GeneID: 29101
• NCBI Accession #: NP_054907
• NCBI GenBank Nucleotide #: NM_014188.2
• Molecular Weight: 16,931 Da
• NCBI Official Full Name: RNA polymerase II subunit A C-terminal domain phosphatase SSU72
• NCBI Official Synonym Full Names: SSU72 homolog, RNA polymerase II CTD phosphatase
• NCBI Official Symbol: SSU72
• NCBI Official Synonym Symbols: HSPC182; PNAS-120
• NCBI Protein Information: RNA polymerase II subunit A C-terminal domain phosphatase SSU72
• UniProt Protein Name: RNA polymerase II subunit A C-terminal domain phosphatase SSU72
• Protein Family: RNA polymerase II subunit A C-terminal domain phosphatase
• UniProt Gene Name: SSU72
• UniProt Synonym Gene Names: CTD phosphatase SSU72

Uniprot Description

Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination. Plays a role in pre-mRNA polyadenylation via its interaction with SYMPK.