Mouse anti-Human SSH2 Monoclonal Antibody | anti-SSH2 antibody

SSH2 (Protein Phosphatase Slingshot Homolog 2, SSH-like Protein 2, SSH2L, SSH-2L, hSSH-2L, KIAA1725) APC

Cat. Num. AAA6139292

• Gene Names: SSH2; SSH-2; SSH-2L
• Reactivity: Human
• Applications: ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SSH2; Monoclonal Antibody; SSH2 (Protein Phosphatase Slingshot Homolog 2; SSH-like Protein 2; SSH2L; SSH-2L; hSSH-2L; KIAA1725) APC; EC=3.1.3.16; EC=3.1.3.48; anti-SSH2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1E11
• Specificity: Recognizes human SSH2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 1423

• Applicable Applications for anti-SSH2 antibody: ELISA (EIA)
• Application Notes: ELISA: 1ng/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa20-118 from human SSH2 (NP_203747) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: ASEADSGEEECRSQPRSISESFLTVKGAALFLPRGNGSSTPRISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQD
• Conjugate: APC
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged SSH2 is ~1ng/ml as a capture antibody.)

Product Categories/Family for anti-SSH2 antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 37674210
• NCBI GeneID: 85464
• NCBI Accession #: NP_203747
• NCBI GenBank Nucleotide #: NM_033389
• UniProt Accession #: Q76I76
• NCBI Official Full Name: protein phosphatase Slingshot homolog 2 isoform 2
• NCBI Official Synonym Full Names: slingshot protein phosphatase 2
• NCBI Official Symbol: SSH2
• NCBI Official Synonym Symbols: SSH-2; SSH-2L
• NCBI Protein Information: protein phosphatase Slingshot homolog 2
• UniProt Protein Name: Protein phosphatase Slingshot homolog 2
• Protein Family: Protein phosphatase Slingshot
• UniProt Gene Name: SSH2
• UniProt Synonym Gene Names: KIAA1725; SSH2L; SSH-2L; hSSH-2L
• UniProt Entry Name: SSH2_HUMAN

NCBI Description

This gene encodes a protein tyrosine phosphatase that plays a key role in the regulation of actin filaments. The encoded protein dephosphorylates and activates cofilin, which promotes actin filament depolymerization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

SSH2: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein. Belongs to the protein-tyrosine phosphatase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; EC 3.1.3.16; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space; cytoskeleton; cytoplasm

Molecular Function: DNA binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; actin binding; phosphoprotein phosphatase activity

Biological Process: regulation of actin polymerization and/or depolymerization; protein amino acid dephosphorylation; regulation of axonogenesis; actin cytoskeleton organization and biogenesis