Mouse SPRY1 Monoclonal Antibody | anti-SPRY1 antibody

SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (Drosophila), hSPRY1) (HRP)

Cat. Num. AAA6182767

• Gene Names: SPRY1; hSPRY1
• Applications: Western Blot
• Purity: Purified
• Synonyms: SPRY1; Monoclonal Antibody; SPRY1 (Sprouty Homolog 1; Antagonist of FGF Signaling (Drosophila); hSPRY1) (HRP); Sprouty Homolog 1; hSPRY1; anti-SPRY1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F1
• Specificity: Recognizes SPRY1.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-SPRY1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: SPRY1 (NP_005832, 1aa-110aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPI
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged SPRY1 is 0.1 ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M02), clone 2F1.Lane 1: SPRY1 transfected lysate (35.1 KDa).Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-SPRY1 antibody

Antibodies; Sprouty

NCBI and Uniprot Product Information

• NCBI GI #: 40538726
• NCBI GeneID: 10252
• NCBI Accession #: NP_005832
• NCBI GenBank Nucleotide #: NM_005841
• UniProt Accession #: O43609
• Molecular Weight: 35kDa
• NCBI Official Full Name: protein sprouty homolog 1
• NCBI Official Synonym Full Names: sprouty RTK signaling antagonist 1
• NCBI Official Symbol: SPRY1
• NCBI Official Synonym Symbols: hSPRY1
• NCBI Protein Information: protein sprouty homolog 1
• UniProt Protein Name: Protein sprouty homolog 1
• Protein Family: Protein sprouty
• UniProt Gene Name: SPRY1
• UniProt Synonym Gene Names: Spry-1
• UniProt Entry Name: SPY1_HUMAN

Uniprot Description

SPRY1: May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Belongs to the sprouty family. Induced by FGF signaling.

Protein type: Motility/polarity/chemotaxis; Inhibitor; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q28.1

Cellular Component: plasma membrane; cytosol

Molecular Function: protein binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of MAP kinase activity; establishment of mitotic spindle orientation; negative regulation of fibroblast growth factor receptor signaling pathway; ureteric bud development; negative regulation of nerve growth factor receptor signaling pathway; induction of an organ; negative regulation of Ras protein signal transduction; metanephros development