Mouse anti-Human SOCS3 Monoclonal Antibody | anti-SOCS3 antibody

Mouse Monoclonal [clone SO1] (IgG2b) to Human SOCS3

Cat. Num. AAA245411

• Gene Names: SOCS3; CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: SOCS3; Monoclonal Antibody; Mouse Monoclonal [clone SO1] (IgG2b) to Human SOCS3; Anti-SOCS3 Antibody (clone SO1) IHC-plus; Cish3; Cytokine-induced SH2 protein 3; CIS-3; CIS3; SSI-3; STAT-induced STAT inhibitor 3; SSI3; ATOD4; SOCS-3; Human SOCS3; anti-SOCS3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: SO1
• Specificity: Recognizes suppressor of cytokine signaling 3 (SOCS3), a 30kD member of the suppressor of cytokine signaling family (SOCS) which negatively regulate Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways. SOCS3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohn's Disease. SOCS3 expression has also been shown to affect IFN alpha sensitivity in tumor studies.
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.4, 0.09% sodium azide. Sourced in TCS.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 225

• Applicable Applications for anti-SOCS3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:100 - 1:500), IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Full - length SOCS3 protein.
• Immunogen Type: Recombinant protein
• Immunogen: SOCS3 antibody was raised against full - length SOCS3 protein.
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SOCS3 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-SOCS3 antibody

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in the negative regulation of cytokines that signal through the JAK/STAT pathway. It inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 45439352
• NCBI GeneID: 9021
• NCBI Accession #: NP_003946.3
• NCBI GenBank Nucleotide #: NM_003955.4
• UniProt Accession #: O14543; O14509
• Molecular Weight: 24,770 Da
• NCBI Official Full Name: suppressor of cytokine signaling 3
• NCBI Official Synonym Full Names: suppressor of cytokine signaling 3
• NCBI Official Symbol: SOCS3
• NCBI Official Synonym Symbols: CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
• NCBI Protein Information: suppressor of cytokine signaling 3; STAT-induced STAT inhibitor 3; cytokine-inducible SH2 protein 3
• UniProt Protein Name: Suppressor of cytokine signaling 3
• Protein Family: Suppressor of cytokine signaling
• UniProt Gene Name: SOCS3
• UniProt Synonym Gene Names: CIS3; SSI3; SOCS-3; CIS-3; SSI-3
• UniProt Entry Name: SOCS3_HUMAN

NCBI Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]

Uniprot Description

SOCS3: SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate recognition component of a SCF-like ECS (Elongin BC- CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. Interacts with multiple activated proteins of the tyrosine kinase signaling pathway including IGF1 receptor, insulin receptor and JAK2. Binding to JAK2 is mediated through the KIR and SH2 domains to a phosphorylated tyrosine residue within the JAK2 JH1 domain. Binds specific activated tyrosine residues of the leptin, EPO, IL12, GSCF and gp130 receptors. Interaction with CSNK1E stabilizes SOCS3 protein. Component of the probable ECS(SOCS3) E3 ubiquitin-protein ligase complex which contains CUL5, RNF7/RBX2, Elongin BC complex and SOCS3. Interacts with CUL5, RNF7, TCEB1 and TCEB2. Interacts with CUL2. Interacts with FGFR3. Interacts with INSR. Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.

Protein type: Inhibitor

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; cytosol

Molecular Function: protein binding; protein kinase inhibitor activity

Biological Process: regulation of growth; cytokine and chemokine mediated signaling pathway; negative regulation of inflammatory response; negative regulation of insulin receptor signaling pathway; protein ubiquitination; negative regulation of protein kinase activity; positive regulation of cell differentiation; JAK-STAT cascade; negative regulation of JAK-STAT cascade; negative regulation of apoptosis

Disease: Dermatitis, Atopic, 4