Mouse anti-Human SNF1LK Monoclonal Antibody | anti-SIK1 antibody

SNF1LK (Serine/Threonine Protein Kinase SIK1, Salt-inducible Protein Kinase 1, SIK-1, Serine/Threonine-protein Kinase SNF1-like Kinase 1, Serine/Threonine-protein Kinase SNF1LK, SIK1, SIK)

Cat. Num. AAA6009804

• Gene Names: SIK1; MSK; SIK; SNF1LK
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SNF1LK; Monoclonal Antibody; SNF1LK (Serine/Threonine Protein Kinase SIK1; Salt-inducible Protein Kinase 1; SIK-1; Serine/Threonine-protein Kinase SNF1-like Kinase 1; Serine/Threonine-protein Kinase SNF1LK; SIK1; SIK); Anti -SNF1LK (Serine/Threonine Protein Kinase SIK1; anti-SIK1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2C12
• Specificity: Recognizes human SIK1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-SIK1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Recombinant corresponding to aa1-101 from human SIK1 with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Testing Data

(Detection limit for recombinant GST tagged SIK1 is 0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-SIK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 116256471
• NCBI GeneID: 150094
• NCBI Accession #: NP_775490.2
• NCBI GenBank Nucleotide #: NM_173354.3
• UniProt Accession #: P57059; Q5R2V5; Q6ZNL8; Q86YJ2; A6NC84
• Molecular Weight: 84,902 Da
• NCBI Official Full Name: serine/threonine-protein kinase SIK1
• NCBI Official Synonym Full Names: salt-inducible kinase 1
• NCBI Official Symbol: SIK1
• NCBI Official Synonym Symbols: MSK; SIK; SNF1LK
• NCBI Protein Information: serine/threonine-protein kinase SIK1; SIK-1; SNF1-like kinase; myocardial SNF1-like kinase; salt-inducible protein kinase 1; serine/threonine protein kinase; serine/threonine-protein kinase SNF1LK; serine/threonine-protein kinase SNF1-like kinase 1
• UniProt Protein Name: Serine/threonine-protein kinase SIK1
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: SIK1
• UniProt Synonym Gene Names: SIK; SNF1LK; SIK-1
• UniProt Entry Name: SIK1_HUMAN

Uniprot Description

SIK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. Interacts with ATP1A1. Interacts (when phosphorylated on Thr-182 and Ser-186) with YWHAZ. Activated by phosphorylation on Thr-182. Also activated by phosphorylation on Thr-322 in response to increases in intracellular sodium in parallel with elevations in intracellular calcium through the reversible sodium/calcium exchanger. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Motility/polarity/chemotaxis; Tumor suppressor; EC 2.7.11.1; CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; cAMP response element binding protein binding; magnesium ion binding; protein kinase binding; ATP binding

Biological Process: protein amino acid autophosphorylation; negative regulation of gluconeogenesis; regulation of cell differentiation; regulation of mitotic cell cycle; rhythmic process; regulation of sodium ion transport; cell cycle; inhibition of CREB transcription factor; protein amino acid phosphorylation; cardiac muscle cell differentiation; entrainment of circadian clock by photoperiod

Disease: Epileptic Encephalopathy, Early Infantile, 30