Mouse anti-Human SNAI1 Monoclonal Antibody | anti-SNAI1 antibody

SNAI1 Antibody

Cat. Num. AAA9602752

• Gene Names: SNAI1; SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity-Chromatography
• Synonyms: SNAI1; Monoclonal Antibody; SNAI1 Antibody; dJ710H13.1; Protein sna; Protein snail homolog 1; Protein snail homolog; SLUGH2; SNA; Sna protein; SNAH; SNAI; snai1; SNAI1_HUMAN; Snail 1 homolog; Snail 1 zinc finger protein; SNAIL; Snail homolog 1 (Drosophila); Drosophila; homolog of; 1; SNAIL1; Zinc finger protein SNAI1; anti-SNAI1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: SNAI1 antibody detects endogenous levels of total SNAI1
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 264

• Applicable Applications for anti-SNAI1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: ELISA: 1:10000; WB: 1:500-1:2000; IF/ICC: 1:100-1:500
• Immunogen: Purified recombinant fragment of human SNAI1 expressed in E. Coli
• Subcellular Location: Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
• Tissue Specificity: Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using SNAI1 mAb against human SNAI1 (AA: 2-264) recombinant protein. (Expected MW is 31.3 kDa) )

Related Product Information for anti-SNAI1 antibody

Description: This protein has many roles during postimplantation development. It is involved in embryonic mesoderm formation and its maintenance and may also be involved in chondrogenesis and in epithelial-mesenchymal inductive interactions.
Function: Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
Subunit Structure: Interacts with FBXL14 and GSK3B. Interacts with BTRC; interaction occurs when it is phosphorylated on the destruction motif. Interacts (via SNAG domain) with WTIP (via LIM domains) (By similarity). Interacts (via SNAG domain) with LIMD1 (via LIM domains), and AJUBA (via LIM domains). Interacts with LOXL2 and LOXL3. Interacts (via N-terminal region) with CSNK2A1. Interacts with EGR1 upon TPA induction. Interacts (via N-terminal region) with LATS2; the interaction is dependent on LATS2 kinase activity but independent of SNAI1 Thr-203 phosphorylation. Interacts (via zinc fingers) with KPNB1 and TNPO1; the interactions mediate nuclear import. Interacts (via zinc fingers) with KPNA1; the interaction disrupts the transport complex with KPNB1 and prevents nuclear import increasing SNAI1 degradation in the cytoplasm. Interacts (via zinc fingers) with KPNA2; the interaction, in combination with KPNB1, mediates nuclear import. Interacts with KPNA4; this interaction mediates nuclear import. May interact (via zinc fingers) with IPO7. Interacts (via zinc fingers) with PARP1; the interaction requires SNAI1 to be poly-ADP-ribosylated and non-phosphorylated (active) by GSK3B. Interacts (via SNAG domain) with KDM1A; the interaction is necessary for the down-regulation of dimethylated H3K4 mark and promoter activity of E-cadherin/CDH1, CDN7 and KRT8. Interacts with TP53/p53 and (via zinc fingers) with NOTCH1 (via intracellular domain); the interactions induce SNAI1 degradation via MDM2-mediated ubiquitination and inhibit SNAI1-induced cell invasion. Interacts with MDM2; the interaction promotes SNAI1 ubiquitination. Interacts (via zinc fingers) with CSNK1E. Interacts with PAK1.
Post-translational Modifications: Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level. Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2. O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein. ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
Similarity: Belongs to the snail C2H2-type zinc-finger protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 18765741
• NCBI GeneID: 6615
• NCBI Accession #: NP_005976.2
• NCBI GenBank Nucleotide #: NM_005985.3
• UniProt Accession #: O95863; Q9P113; Q9UBP7; Q9UHH7; B2R842
• Molecular Weight: Observed: 29 kDa; Predicted: 30 kDa
• NCBI Official Full Name: zinc finger protein SNAI1
• NCBI Official Synonym Full Names: snail family transcriptional repressor 1
• NCBI Official Symbol: SNAI1
• NCBI Official Synonym Symbols: SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1
• NCBI Protein Information: zinc finger protein SNAI1
• UniProt Protein Name: Zinc finger protein SNAI1
• Protein Family: Protein snail
• UniProt Gene Name: SNAI1
• UniProt Synonym Gene Names: SNAH; Protein sna

NCBI Description

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (). Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.