Western Blot (WB)
(Western Blot detection against Immunogen (72.64kD).)
Mouse anti-Human SMPD2 Monoclonal Antibody | anti-Smpd2 antibody
SMPD2 (Sphingomyelin Phosphodiesterase 2, Lyso-platelet-activating Factor-phospholipase C, Lyso-PAF-PLC, N-SMase, nSMase, Neutral Sphingomyelinase)
Gene Names
Smpd2; nSMase
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
SMPD2; Monoclonal Antibody; SMPD2 (Sphingomyelin Phosphodiesterase 2; Lyso-platelet-activating Factor-phospholipase C; Lyso-PAF-PLC; N-SMase; nSMase; Neutral Sphingomyelinase); Anti -SMPD2 (Sphingomyelin Phosphodiesterase 2; anti-Smpd2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG3,k
Clone Number
2C9
Specificity
Recognizes human SMPD2.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAAHHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAEYNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGSEEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHSGTPLSDHEALMATLFVRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGAGEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRTKEQ
Applicable Applications for anti-Smpd2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Optimal dilutions to be detemined by the researcher.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Optimal dilutions to be detemined by the researcher.
Immunogen
Full length recombinant corresponding to aa1-424 from human SMPD2 (AAH00038) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (72.64kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (72.64kD).)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to SMPD2 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged SMPD2 is ~10ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged SMPD2 is ~10ng/ml as a capture antibody.)
Related Product Information for anti-Smpd2 antibody
SMPD2 converts sphingomyelin to ceramide and hydrolyzes 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
Product Categories/Family for anti-Smpd2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,645 Da
NCBI Official Full Name
sphingomyelin phosphodiesterase 2
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 2, neutral membrane
NCBI Official Symbol
Smpd2
NCBI Official Synonym Symbols
nSMase
NCBI Protein Information
sphingomyelin phosphodiesterase 2; N-SMase; lyso-PAF-PLC; neutral sphingomyelinase; lyso-platelet-activating factor-phospholipase C
UniProt Protein Name
Sphingomyelin phosphodiesterase 2
UniProt Gene Name
Smpd2
UniProt Synonym Gene Names
Lyso-PAF-PLC; N-SMase; nSMase
UniProt Entry Name
NSMA_RAT
NCBI Description
catalyzes the Mg2+ dependent hydrolysis of sphingomyelin to form N-acylsphingosine and choline phosphate; also has a lower activity for the hydrolysis of lyso-platelet activating factor to 1-alkylglycerol [RGD, Feb 2006]
Uniprot Description
Function: Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
Catalytic activity: Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.
Cofactor: Magnesium.
Pathway: Lipid metabolism; sphingolipid metabolism.
Subcellular location: Membrane; Multi-pass membrane protein.
Sequence similarities: Belongs to the neutral sphingomyelinase family.
Research Articles on Smpd2
Similar Products
Product Notes
The Smpd2 smpd2 (Catalog #AAA648823) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMPD2 (Sphingomyelin Phosphodiesterase 2, Lyso-platelet-activating Factor-phospholipase C, Lyso-PAF-PLC, N-SMase, nSMase, Neutral Sphingomyelinase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SMPD2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be detemined by the researcher. Researchers should empirically determine the suitability of the Smpd2 smpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKPNFSLRLR IFNLNCWGIP YLSKHRADRM RRLGDFLNQE SFDLALLEEV WSEQDFQYLR QKLSPTYPAA HHFRSGIIGS GLCVFSKHPI QELTQHIYTL NGYPYMIHHG DWFSGKAVGL LVLHLSGMVL NAYVTHLHAE YNRQKDIYLA HRVAQAWELA QFIHHTSKKA DVVLLCGDLN MHPEDLGCCL LKEWTGLHDA YLETRDFKGS EEGNTMVPKN CYVSQQELKP FPFGVRIDYV LYKAVSGFYI SCKSFETTTG FDPHSGTPLS DHEALMATLF VRHSPPQQNP SSTHGPAERS PLMCVLKEAW TELGLGMAQA RWWATFASYV IGLGLLLLAL LCVLAAGGGA GEAAILLWTP SVGLVLWAGA FYLFHVQEVN GLYRAQAELQ HVLGRAREAQ DLGPEPQPAL LLGQQEGDRT KEQ. It is sometimes possible for the material contained within the vial of "SMPD2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
