Mouse anti-Human sMD-2 and sMD-2/TLR4 Monoclonal Antibody | anti-sMD-2 and sMD-2/TLR4 antibody
sMD-2 and sMD-2/TLR4, Human, mAb 4H1, FITC
Reactivity
Human
Applications
Immunofluorescence, Western Blot
Synonyms
sMD-2 and sMD-2/TLR4; Monoclonal Antibody; Human; mAb 4H1; FITC; Lymphocyte antigen 96; ESOP-1; LY96; anti-sMD-2 and sMD-2/TLR4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
1 ml (100 ug/ml) 0.2 um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide
Applicable Applications for anti-sMD-2 and sMD-2/TLR4 antibody
FS, Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Application Use: For immuno assay, Western blotting and immunofluorescence dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Conjugate
FITC
Immunogen
Baculovirally expressed His-tagged human MD-2
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
Related Product Information for anti-sMD-2 and sMD-2/TLR4 antibody
Product Description: The monoclonal antibody 4H1 reacts with soluble MD-2 (sMD-2) and the sMD-2/Toll-like receptor 4 (TLR4, CD284) complex. TLRs belong to a family of proteins that specifically recognizes and senses microbial products. They are highly conserved throughout evolution and act as innate immune recognition receptors against many pathogens. TLR4 is a functional receptor for gram-negative bacterial lipopolysaccharides (LPS). TLR4 associates with MD-2 which is absolutely required for LPS-induced activation of TLR4.
MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomers and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.
The monoclonal antibody 4H1 reacts with both the monomeric and the polymeric form of sMD-2. In addition, the monoclonal antibody 4H1 is able to inhibit LPS binding to MD-2.It does not cross-react with mouse MD-2.
MD-2 exists as a cell surface protein in association with TLR4. It also exists as secreted forms consisting of MD-2 monomers and multimers. Circulating sMD-2 is mainly present as a doublet of ~20 and 25 kD, representing differentially glycosylated forms. Unlike TLR4, sMD-2 binds directly LPS without the need of soluble CD14 (sCD14). However, LPS-MD-2 interactions are increased when LPS is pretreated with CD14. Only monomeric sMD-2 is biologically active and able to associate with TLR4 and LPS. sMD-2 circulates in plasma of healthy individuals as a non-active, polymeric protein. In septic plasma, the total amount of sMD-2 was strongly elevated and contained both sMD-2 polymers and monomers. Soluble MD-2 is proposed to be an important mediator of organ inflammation during sepsis. During experimental human endotoxemia, the monomeric and total sMD-2 content in plasma increased with the kinetics of an acute phase protein. This parallels enhanced TLR4 costimulatory activity. In vitro studies revealed that sMD-2 release appears to be restricted to endothelial and dendritic cells.
The monoclonal antibody 4H1 reacts with both the monomeric and the polymeric form of sMD-2. In addition, the monoclonal antibody 4H1 is able to inhibit LPS binding to MD-2.It does not cross-react with mouse MD-2.
References
1. Viriyakosol, S et al; Characterization of monoclonal antibodies to human soluble MD-2 protein. Hybridoma 2006, 25: 349
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Product Notes
The sMD-2 and sMD-2/TLR4 (Catalog #AAA584274) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The sMD-2 and sMD-2/TLR4, Human, mAb 4H1, FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's sMD-2 and sMD-2/TLR4 can be used in a range of immunoassay formats including, but not limited to, FS, Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB). Application Use: For immuno assay, Western blotting and immunofluorescence dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the sMD-2 and sMD-2/TLR4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "sMD-2 and sMD-2/TLR4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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