Mouse anti-Human SIRP alpha/CD172a Monoclonal Antibody | anti-SIRP alpha/CD172a antibody

Mouse anti Human SIRP alpha/CD172a Monoclonal Antibody

Cat. Num. AAA2543628

• Gene Names: SIRPA; BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Synonyms: SIRP alpha/CD172a; Monoclonal Antibody; Mouse anti Human SIRP alpha/CD172a Monoclonal Antibody; anti-SIRP alpha/CD172a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Sequence Length: 503

• Applicable Applications for anti-SIRP alpha/CD172a antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB: 10-20 mug/mL; ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect Human SIRPA. The detection limit for Human SIRPA is 0.0195 ng/well.; Flow Cytometry: 0.5-2 mug/Test
• Immunogen: Recombinant Human SIRPA protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 49899261
• NCBI GeneID: 140885
• Molecular Weight: 54,852 Da
• NCBI Official Full Name: Signal-regulatory protein alpha
• NCBI Official Synonym Full Names: signal regulatory protein alpha
• NCBI Official Symbol: SIRPA
• NCBI Official Synonym Symbols: BIT; MFR; P84; SIRP; MYD-1; SHPS1; CD172A; PTPNS1
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type substrate 1
• UniProt Gene Name: SIRPA
• UniProt Synonym Gene Names: BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP; SHP substrate 1; SHPS-1; Bit; Sirp-alpha-1; Sirp-alpha-2; Sirp-alpha-3
• UniProt Entry Name: SHPS1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SHPS1: Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB. Binds PTK2B. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: membrane; plasma membrane

Biological Process: cell adhesion; leukocyte migration