Immunoflurescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(BF8008) and rabbit anti-beta tubulin Ab(AF7011) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Mouse SIGLEC15 Monoclonal Antibody | anti-SIGLEC15 antibody
SIGLEC15 Mouse Monoclonal Antibody
Gene Names
SIGLEC15; CD33L3; HsT1361; SIGLEC-15
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
Affinity-chromatography.
Synonyms
SIGLEC15; Monoclonal Antibody; SIGLEC15 Mouse Monoclonal Antibody; CD33 antigen-like 3; CD33 molecule-like 3; CD33L3; HsT1361; Sialic acid-binding Ig-like lectin 15; SIG15_HUMAN; Siglec-15; anti-SIGLEC15 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
AFB19405
Specificity
SIGLEC15 Mouse Monoclonal Antibody detects endogenous levels of total SIGLEC15.
Purity/Purification
Affinity-chromatography.
Form/Format
Liquid. Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SIGLEC15 antibody
Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
IHC: 1:50-1:200
ELISA(peptide): 1:20000-1:40000
IF/ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human SIGLEC15.
Conjugation
Unconjugated
Similarity
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Subunit Structure
Interacts with TYROBP and HCST.
Tissue Specificity
Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.
Function
Binds sialylated glycoproteins.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunoflurescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(BF8008) and rabbit anti-beta tubulin Ab(AF7011) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunoflurescence (IF)/Immunocytochemistry (ICC)
(Staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(BF8008) and rabbit anti-beta tubulin Ab(AF7011) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-mouse IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-rabbit IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/100 staining mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Product Categories/Family for anti-SIGLEC15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,086 Da
NCBI Official Full Name
sialic acid-binding Ig-like lectin 15
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 15
NCBI Official Symbol
SIGLEC15
NCBI Official Synonym Symbols
CD33L3; HsT1361; SIGLEC-15
NCBI Protein Information
sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3
UniProt Protein Name
Sialic acid-binding Ig-like lectin 15
Protein Family
UniProt Gene Name
SIGLEC15
UniProt Synonym Gene Names
CD33L3; Siglec-15
UniProt Entry Name
SIG15_HUMAN
Uniprot Description
CD33L3: Binds sialylated glycoproteins. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q12.3
Cellular Component: protein complex; integral to membrane; plasma membrane
Biological Process: regulation of bone resorption; regulation of actin cytoskeleton organization and biogenesis; innate immune response
Research Articles on SIGLEC15
Similar Products
Product Notes
The SIGLEC15 siglec15 (Catalog #AAA9629027) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SIGLEC15 Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIGLEC15 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). IHC: 1:50-1:200 ELISA(peptide): 1:20000-1:40000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SIGLEC15 siglec15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGLEC15, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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