Mouse SHC1 Monoclonal Antibody | anti-SHC1 antibody

SHC1 antibody

Cat. Num. AAA838692

• Gene Names: SHC1; SHC; SHCA
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: SHC1 antibody was purified by affinity chromatography.
• Synonyms: SHC1; Monoclonal Antibody; SHC1 antibody; Monoclonal SHC1; Anti-SHC1; SHC transforming protein 1; SHC; SHCA; SH2 domain protein C1; SHC-transforming protein 3; SHC-transforming protein A; SH 1c; SH-1c; anti-SHC1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A1
• Purity/Purification: SHC1 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 369

• Applicable Applications for anti-SHC1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; IHC: 1:50; WB: 1:2000
• Biological Significance: This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effectsof reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix.
• Cross-Reactivity: Human
• Immunogen: SHC1 antibody was raised in mouse using a full length recombinant protein of human SHC1 (NP_003020) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant SHC1 protein using SHC1 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SHC1 protein (Right) or empty vector (Left) detected with SHC1 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of SHC1 protein in paraffin embedded Human colon tissue using SHC1 antibody)

Related Product Information for anti-SHC1 antibody

Mouse monoclonal SHC1 antibody

Product Categories/Family for anti-SHC1 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 21706900
• NCBI GeneID: 6464
• Molecular Weight: 51,682 Da
• NCBI Official Full Name: SHC1 protein
• NCBI Official Synonym Full Names: SHC (Src homology 2 domain containing) transforming protein 1
• NCBI Official Symbol: SHC1
• NCBI Official Synonym Symbols: SHC; SHCA
• NCBI Protein Information: SHC-transforming protein 1
• UniProt Protein Name: SHC-transforming protein 1
• Protein Family: SHC-transforming protein
• UniProt Gene Name: SHC1
• UniProt Synonym Gene Names: SHC; SHCA; SH2 domain protein C1
• UniProt Entry Name: SHC1_HUMAN

NCBI Description

This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

SHC1: an adaptor protein containing a SH2 domain and a PID domain within a PH domain-like fold. Couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Six human isoforms are produced by alternative promoter usage and alternative splicing. Isoforms p66, p52 and p46 (P29353-1, -2, and -3), produced by alternative initiation, variously regulate growth factor signaling, oncogenesis, intracellular oxidant levels, and apoptosis. Isoforms p46 and p52, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex, thus initiating the cytoplasmic proliferative Ras signaling cascade in various non-neuronal systems. Isoform p66 does not mediate Ras activation, but associates with mitochondria where it controls intracellular redox status, mitochondrial permeability, life span, and stress-induced apoptosis. p66 acts as a downstream target of the tumor suppressor p53 and is required for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. P66 deletion in mice decreases the incidence of aging-associated diseases, such as atherosclerosis, and significantly prolongs life span. Participates in signaling downstream of TIE2, the tyrosine kinase receptor for angiopoietin, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis. Interacts with tyrosine-phosphorylated CD3T, DDR2, LRP1, IRS4, SHP, FLT4, PDGFRB, TIE2, TrkA, -B and -C. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. p66Shc is known to be activated by the mutant SOD1 associated with familial forms of amyotrophic lateral sclerosis (ALS), causing a decrease in the activity of Rac1 through a redox-sensitive regulation. In case of oxidative conditions, phosphorylation at S36 of isoform p66Shc, leads to mitochondrial accumulation p66 plays a role in mediating mitophagy and determining neuronal cell fate following acute oxygen glucose deprivation. Isoform p46 is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.

Protein type: Mitochondrial; Adaptor/scaffold; Apoptosis; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: mitochondrial matrix; plasma membrane; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; ephrin receptor binding; neurotrophin TRKA receptor binding; protein-tyrosine kinase activity; phospholipid binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; protein kinase binding; insulin receptor binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; unfolded protein response; activation of MAPK activity; heart development; regulation of epidermal growth factor receptor activity; MAPKKK cascade; cell-cell adhesion; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of growth; Ras protein signal transduction; actin cytoskeleton reorganization; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; angiogenesis; blood coagulation; leukocyte migration; positive regulation of DNA replication