Mouse anti-Human SH3BP5 Monoclonal Antibody | anti-SH3BP5 antibody

SH3BP5 (SH3 Domain-binding Protein 5, SH3BP-5, SH3 Domain-binding Protein That Preferentially Associates with BTK, SAB)

Cat. Num. AAA6001538

• Gene Names: SH3BP5; SAB; SH3BP-5
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SH3BP5; Monoclonal Antibody; SH3BP5 (SH3 Domain-binding Protein 5; SH3BP-5; SH3 Domain-binding Protein That Preferentially Associates with BTK; SAB); Anti -SH3BP5 (SH3 Domain-binding Protein 5; anti-SH3BP5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2B3
• Specificity: Recognizes human SH3BP5.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.

• Applicable Applications for anti-SH3BP5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: Immunofluorescence: 10ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
• Immunogen: Recombinant corresponding to aa4-113 from human SH3BP5 with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(SH3BP5 monoclonal antibody Western Blot analysis of SH3BP5 expression in A-431.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to SH3BP5 on HeLa cell (antibody concentration 10ug/ml).)

Testing Data

(Detection limit for recombinant GST tagged SH3BP5 is approximately 0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-SH3BP5 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 109134344
• NCBI GeneID: 9467
• NCBI Accession #: NP_001018009.2
• NCBI GenBank Nucleotide #: NM_001018009.3
• UniProt Accession #: O60239; Q5JWV9; B3KQW6
• Molecular Weight: 50,425 Da
• NCBI Official Full Name: SH3 domain-binding protein 5 isoform b
• NCBI Official Synonym Full Names: SH3-domain binding protein 5 (BTK-associated)
• NCBI Official Symbol: SH3BP5
• NCBI Official Synonym Symbols: SAB; SH3BP-5
• NCBI Protein Information: SH3 domain-binding protein 5; SH3 domain-binding protein that preferentially associates with BTK
• UniProt Protein Name: SH3 domain-binding protein 5
• Protein Family: SH3 domain-binding protein
• UniProt Gene Name: SH3BP5
• UniProt Synonym Gene Names: SAB; SH3BP-5
• UniProt Entry Name: 3BP5_HUMAN

Uniprot Description

SH3BP5: Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death. Belongs to the SH3BP5 family. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 3p24.3

Cellular Component: mitochondrion; cytoplasm

Molecular Function: protein binding; protein kinase inhibitor activity; SH3 domain binding

Biological Process: negative regulation of protein kinase activity; signal transduction