Mouse anti-Human SFPQ Monoclonal Antibody | anti-SFPQ antibody

Mouse Monoclonal [clone 6D7] (IgG2a,k) to Human SFPQ

Cat. Num. AAA246508

• Gene Names: SFPQ; PSF; POMP100; PPP1R140
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: SFPQ; Monoclonal Antibody; Mouse Monoclonal [clone 6D7] (IgG2a; k) to Human SFPQ; Anti-SFPQ Antibody (clone 6D7) IHC-plus; 100 kDa DNA-pairing protein; HPOMp100; PTB-associated splicing factor; PSF; POMP100; PTB-associated-splicing factor; Human SFPQ; anti-SFPQ antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6D7
• Specificity: Human SFPQ
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 707

• Applicable Applications for anti-SFPQ antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: SFPQ (NP_005057, 269 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: SFPQ antibody was raised against sFPQ (NP_005057, 269 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C or lower. Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SFPQ antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to SFPQ on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(SFPQ monoclonal antibody clone 6D7 Western blot of SFPQ expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SFPQ is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4826998
• NCBI GeneID: 6421
• NCBI Accession #: NP_005057.1
• NCBI GenBank Nucleotide #: NM_005066.2
• UniProt Accession #: P23246; P30808; Q5SZ71
• Molecular Weight: 72,263 Da
• NCBI Official Full Name: splicing factor, proline- and glutamine-rich
• NCBI Official Synonym Full Names: splicing factor proline/glutamine-rich
• NCBI Official Symbol: SFPQ
• NCBI Official Synonym Symbols: PSF; POMP100; PPP1R140
• NCBI Protein Information: splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract-binding protein-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated)
• UniProt Protein Name: Splicing factor, proline- and glutamine-rich
• Protein Family: Splicing factor
• UniProt Gene Name: SFPQ
• UniProt Synonym Gene Names: PSF; hPOMp100; PSF; PTB-associated-splicing factor
• UniProt Entry Name: SFPQ_HUMAN

Uniprot Description

PSF: a proline-and glutamine-rich splicing factor. Essential pre-mRNA splicing factor required early in spliceosome formation. Seems to also bind DNA. The active complex is a heterotetramer of two 52 kDa and two 100 kDa subunits. Reported to be a PKC-alpha-binding protein in the cell nucleus. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; RNA-binding; RNA splicing

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; chromatin; nucleus; paraspeckles

Molecular Function: protein binding; histone deacetylase binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; mRNA processing; DNA repair; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; DNA recombination