Mouse anti-Human SERPING1 Monoclonal Antibody | anti-SERPING1 antibody

SERPING1 (Serine/Cysteine Proteinase Inhibitor, Clade G, Member 1, Complement C1 Inhibitor, C1-INH, HAE1, HAE2)

Cat. Num. AAA602040

• Gene Names: SERPING1; C1IN; C1NH; HAE1; HAE2; C1INH
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: SERPING1; Monoclonal Antibody; SERPING1 (Serine/Cysteine Proteinase Inhibitor; Clade G; Member 1; Complement C1 Inhibitor; C1-INH; HAE1; HAE2); Anti -SERPING1 (Serine/Cysteine Proteinase Inhibitor; anti-SERPING1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7H118
• Specificity: Recognizes human SERPING1. In Western Blot, does not crossreact with recombinant human SERPINA1, -A3, -A4, -A5, -C1, -D1, -E2 or -F1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-SERPING1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Direct ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1ug/ml; Immunoprecipitation: 25ug/ml to immunoprecipitate from conditioned cell culture medium spiked with human SERPINGG1.; Optimal dilutions should be determined by the researcher.
• Immunogen: Human plasma-derived SERPING1.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with MBS602040 (1ug/ml) followed by an IgG (HRP) Anti-Mouse IgG Secondary Antibody. A specific band was detected for Serpin G1/C1 Inhibitor at ~100kD (as indicated). This experiment was conducted under reducing conditions and using 25mM Tris, pH 7.4, 0.15M NaCl, 0.1% Tween 20.)

Related Product Information for anti-SERPING1 antibody

As a member of the serpin superfamily of serine protease inhibitors, SERPING1 is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. In addition, it inhibits plasma kallikrein and coagulation factor XIIa, two serine proteases involved in the processing of kininogen to release bradykinin. Therefore, it plays an important role in regulating activation of both the complement and contact systems (1). SERPING1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa (2). The deduced amino acid sequence of human Serpin G1 precursor consists of 500 residues with a signal peptide. The mature protein of 478 amino acid residues is heavily glycosylated (1).

Product Categories/Family for anti-SERPING1 antibody

Antibodies; Abs to Protease Inhibitors

NCBI and Uniprot Product Information

• NCBI GI #: 54300698
• NCBI GeneID: 710
• UniProt Accession #: Q5UGI5; P05155; Q5UGI6
• Molecular Weight: 14,110 Da
• NCBI Official Full Name: serine/cysteine proteinase inhibitor clade G member 1 splice variant 3
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• NCBI Official Symbol: SERPING1
• NCBI Official Synonym Symbols: C1IN; C1NH; HAE1; HAE2; C1INH
• NCBI Protein Information: plasma protease C1 inhibitor; serpin G1; OTTHUMP00000197003; OTTHUMP00000197004; OTTHUMP00000197007; OTTHUMP00000235286; OTTHUMP00000235287; C1-inhibiting factor; C1 esterase inhibitor; complement component 1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1
• UniProt Protein Name: Serine/cysteine proteinase inhibitor clade G member 1 splice variant 3
• Protein Family: Plasma protease C1 inhibitor
• UniProt Gene Name: SERPING1
• UniProt Entry Name: Q5UGI5_HUMAN

NCBI Description

This gene encodes a highly glycosylated plasma protein involved in the regulation of the complement cascade. Its protein inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq]

Uniprot Description

SERPING1: a protein protease inhibitor (C1-inhibitor) that forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play an important role in regulating complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Mutations of the SERPING1 gene is associated with adult macular degeneration can also cause hereditary angioedema. Binds to E.coli stcE which allows localization of SERPING1 to cell membranes thus protecting the bacteria against complement-mediated lysis. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; fibrinolysis; platelet degranulation; blood circulation; innate immune response; blood coagulation; blood coagulation, intrinsic pathway; complement activation, classical pathway; aging

Disease: Complement Component 4, Partial Deficiency Of; Angioedema, Hereditary, Type I