Western Blot (WB)
(Western Blot: Sample: Recombinant SEMA5B, Human.)
Mouse anti-Human Semaphorin 5B (SEMA5B) Monoclonal Antibody | anti-SEMA5B antibody
Monoclonal Antibody to Semaphorin 5B (SEMA5B)
Gene Names
SEMA5B; SemG; SEMAG
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Semaphorin 5B (SEMA5B); Monoclonal Antibody; Monoclonal Antibody to Semaphorin 5B (SEMA5B); anti-SEMA5B antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
D4
Specificity
The antibody is a mouse monoclonal antibody raised against SEMA5B. It has been selected for its ability to recognize SEMA5B in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- LLSLV RGLLPCLPPG ARTAEGPIMV LAGPLAVSLL LPSLTLLVSH LSSSQDVSSE PSSEQQLCAL SKHPTVAFED LQPWVSNFTY PGARDFSQLA LDPSGNQLIV
MGHHHHHHSGSEF- LLSLV RGLLPCLPPG ARTAEGPIMV LAGPLAVSLL LPSLTLLVSH LSSSQDVSSE PSSEQQLCAL SKHPTVAFED LQPWVSNFTY PGARDFSQLA LDPSGNQLIV
Sequence Length
1057
Applicable Applications for anti-SEMA5B antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant SEMA5B (Leu36~Glu161) expressed in E.coli.
Cross Reactivity
Human
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant SEMA5B, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SEMA5B, Human.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
131,904 Da
NCBI Official Full Name
semaphorin-5B isoform 1
NCBI Official Synonym Full Names
semaphorin 5B
NCBI Official Symbol
SEMA5B
NCBI Official Synonym Symbols
SemG; SEMAG
NCBI Protein Information
semaphorin-5B
UniProt Protein Name
Semaphorin-5B
Protein Family
UniProt Gene Name
SEMA5B
UniProt Synonym Gene Names
KIAA1445; SEMAG
NCBI Description
This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
SEMA5B: a single-pass type I membrane protein of the semaphorin family that may act as positive axonal guidance cues. Two alternatively spliced human isoforms have been described.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q21.1
Cellular Component: extracellular space; integral component of membrane
Biological Process: axon extension; detection of light stimulus involved in visual perception; negative chemotaxis
Research Articles on SEMA5B
1. SEMA5B could possibly serve as a candidate gene for alterations associated with asbestos exposure.
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Product Notes
The SEMA5B sema5b (Catalog #AAA2012805) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Semaphorin 5B (SEMA5B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Semaphorin 5B (SEMA5B) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SEMA5B sema5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- LLSLV RGLLPCLPPG ARTAEGPIMV LAGPLAVSLL LPSLTLLVSH LSSSQDVSSE PSSEQQLCAL SKHPTVAFED LQPWVSNFTY PGARDFSQLA LDPSGNQLIV. It is sometimes possible for the material contained within the vial of "Semaphorin 5B (SEMA5B), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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