ELISA (EIA)
(Sandwich ELISA analysis of human SAP using Mouse anti Human SAP as a capture reagent and biotinylated Mouse anti Human SAP (MBS226228) as a detection reagent with purified native sap as the antigen. Detection was performed using HRP conjugated )
Mouse anti-Human SAP Monoclonal Antibody | anti-SAP antibody
Mouse anti Human SAP:Biotin
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Purified IgG conjugated to Biotin - liquid
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
ELISA (EIA)
(Sandwich ELISA analysis of human SAP using Mouse anti Human SAP as a capture reagent and biotinylated Mouse anti Human SAP (MBS226228) as a detection reagent with purified native sap as the antigen. Detection was performed using HRP conjugated )
ELISA (EIA)
(Sandwich ELISA analysis of human SAP using Mouse anti Human SAP as a capture reagent and biotinylated Mouse anti Human SAP (MBS226228) as a detection reagent with purified native sap as the antigen. Detection was performed using HRP conjugated )
SAP is able to bind to a variety of molecules including apoptotic debris, bacterial polysaccharides, amyloid deposits and bacterial toxins. Phagocytic cells such as monocytes and macrophages then bind the SAP and engulf the material bound to SAP as part of debris clearance (Pilling & Gomer 2018).
The biotinylated Mouse anti Human SAP antibody, clone AB12-5A1 can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human SAP antibody, clone AB18-4E2 as the capture antibody.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a glycoprotein, belonging to the pentraxin family of proteins, which has a characteristic pentameric organization. These family members have considerable sequence homology which is thought to be the result of gene duplication. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo. [provided by RefSeq, Sep 2008]
Uniprot Description
APCS: Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. Belongs to the pentaxin family.
Protein type: Secreted, signal peptide; Secreted; Chaperone
Chromosomal Location of Human Ortholog: 1q21-q23
Cellular Component: extracellular matrix; extracellular space; protein complex; extracellular region; nucleus
Molecular Function: complement component C1q binding; unfolded protein binding; virion binding; calcium ion binding; carbohydrate binding
Biological Process: protein folding; negative regulation of virion penetration into host cell; acute-phase response; negative regulation of acute inflammatory response; innate immune response; chaperone-mediated protein complex assembly; negative regulation of monocyte differentiation; negative regulation of viral reproduction
Research Articles on SAP
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Product Notes
The SAP apcs (Catalog #AAA226228) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human SAP:Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SAP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). ELISA: 1.0ug/ml. Researchers should empirically determine the suitability of the SAP apcs for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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