Mouse SAFB1 / SAFB Monoclonal Antibody | anti-SAFB1 antibody

Mouse Monoclonal [clone 6F7] (IgG1) to Human SAFB1 / SAFB

Cat. Num. AAA245086

• Gene Names: SAFB; HAP; HET; SAFB1; SAF-B1
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: SAFB1 / SAFB; Monoclonal Antibody; Mouse Monoclonal [clone 6F7] (IgG1) to Human SAFB1 / SAFB; Anti-SAFB1 / SAFB Antibody (aa345-357; clone 6F7) IHC-plus; SAFB; HAP; HET; Scaffold attachment factor B; SAB-B1; SAFB1; SAF-B; SAF-B1; Scaffold attachment factor B1; Hsp27 ERE-TATA binding protein; HSP27 ERE-TATA-binding protein; Human SAFB1; anti-SAFB1 antibody

• Host: Mouse
• Reactivity: Gibbon, Gorilla, Human, Monkey, Orangutan
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6F7
• Specificity: A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human HET protein.
• Purity/Purification: Protein G Purified
• Form/Format: PBS. Sourced in TCS.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 915

• Applicable Applications for anti-SAFB1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF, IHC-P (5 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human SAFB. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: SAFB1 / SAFB antibody was raised against synthetic peptide from human SAFB. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset (100%).
• Antigen Modification: aa345-357
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-SAFB antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 21264343
• NCBI GeneID: 6294
• NCBI Accession #: NP_002958.2
• NCBI GenBank Nucleotide #: NM_002967.3
• UniProt Accession #: Q15424; O60406; Q59HH8; A0AV56; B7Z5B6; B7ZLP6; F5H0H3
• Molecular Weight: 102,768 Da
• NCBI Official Full Name: scaffold attachment factor B1 isoform 3
• NCBI Official Synonym Full Names: scaffold attachment factor B
• NCBI Official Symbol: SAFB
• NCBI Official Synonym Symbols: HAP; HET; SAFB1; SAF-B1
• NCBI Protein Information: scaffold attachment factor B1; SAF-B; SAB-B1; HSP27 ERE-TATA-binding protein; Hsp27 ERE-TATA binding protein; glutathione S-transferase fusion protein; HSP27 estrogen response element-TATA box-binding protein; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein
• UniProt Protein Name: Scaffold attachment factor B1
• UniProt Gene Name: SAFB
• UniProt Synonym Gene Names: HAP; HET; SAFB1; SAF-B; SAF-B1; HSP27 ERE-TATA-binding protein
• UniProt Entry Name: SAFB1_HUMAN

NCBI Description

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Uniprot Description

SAFB1: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. Can inhibit cell proliferation.

Protein type: Nuclear receptor co-regulator; RNA-binding; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; double-stranded DNA binding; nucleotide binding; chromatin binding

Biological Process: establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; transcription, DNA-dependent; hormone metabolic process; positive regulation of transcription from RNA polymerase II promoter; growth