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Rat anti-Mouse S100A9 Monoclonal Antibody | anti-S100A9 antibody

S100A9, Mouse, mAb MU14-2A5

Reactivity
Mouse
Applications
Immunofluorescence, Western Blot
Synonyms
S100A9; Monoclonal Antibody; Mouse; mAb MU14-2A5; Myeloid-related protein 14; MRP 14 or Calgranulin B; anti-S100A9 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1
Clone Number
MU14-2A5
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Sequence Length
114
Applicable Applications for anti-S100A9 antibody
Frozen sections (F), Functional Studies (FS), Immuno Assay (IA), Immunofluorescence (IF), Paraffin sections (P), Western Blot (WB)
Application Notes
W: A reduced sample treatment and SDS-Page was used. The band size is 13-15 kDa.
For immunohistochemistry and Western blotting], dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting.
Positive Control
Mouse bone marrow cell extracts
Immunogen
Complete mouse S100A9 protein
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Related Product Information for anti-S100A9 antibody
The monoclonal antibody MU14-2A5 recognizes S100A9. The calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. MRP14 is co-expressed with human MRP8, a homologue protein in myeloid cells, and plays an essential role in calcium dependent functions during inflammation. This role includes the activation of Mac-1, the beta2 integrin which is involved in neutrophil adhesion to endothelial cells. MRP14 is distinguished from other S100 member proteins by its long C-terminal region. The expression of MRP-8 (S100A8) and MRP-14 (S100A9) is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. In inflammatory conditions small venueles stain with both anti-S100A8 and S100A9. The staining of the two subunits is always coincident. The S100A8/A9 complex has antibacterial, antifungal and immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils.

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
13,242 Da
NCBI Official Full Name
S100A9, partial
UniProt Protein Name
Protein S100-A9
Protein Family
UniProt Gene Name
S100A9
UniProt Synonym Gene Names
CAGB; CFAG; MRP14; MRP-14; p14
UniProt Entry Name
S10A9_HUMAN

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; nucleus; cytosol

Molecular Function: arachidonic acid binding; antioxidant activity; RAGE receptor binding; signal transducer activity; protein binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chronic inflammatory response; chemokine production; cytokine production; positive regulation of peptide secretion; response to lipopolysaccharide; signal transduction; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; cell-cell signaling; response to zinc ion; actin cytoskeleton reorganization; defense response to bacterium; innate immune response; autophagy; regulation of integrin biosynthetic process; inflammatory response; defense response to fungus; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis

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Product Notes

The S100A9 s100a9 (Catalog #AAA584697) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The S100A9, Mouse, mAb MU14-2A5 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's S100A9 can be used in a range of immunoassay formats including, but not limited to, Frozen sections (F), Functional Studies (FS), Immuno Assay (IA), Immunofluorescence (IF), Paraffin sections (P), Western Blot (WB). W: A reduced sample treatment and SDS-Page was used. The band size is 13-15 kDa. For immunohistochemistry and Western blotting], dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Researchers should empirically determine the suitability of the S100A9 s100a9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "S100A9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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