Mouse S100A9 Monoclonal Antibody | anti-S100A9 antibody

S100A9 (Macrophage Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439309

• Gene Names: S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• Reactivity: Human and Rat. Others not tested.
• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry
• Synonyms: S100A9; Monoclonal Antibody; S100A9 (Macrophage Marker) Mouse Monoclonal Antibody; S100-A9; MRP14; calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14; S100 calcium-binding protein A9 (calgranulin B); anti-S100A9 antibody

• Host: Mouse
• Reactivity: Human and Rat. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 47-8D3
• Specificity: This MAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% azide. Also available WITHOUT azide at 1.0mg/ml.

• Applicable Applications for anti-S100A9 antibody: Flow Cytometry (FC), Immunofluorescence (IF), Immunohistology (Formalin-fixed)
• Application Notes: Flow Cytometry: 0.5-1ug/million cells; Immunofluorescence: 0.5-1ug/ml; Immunohistology (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT; (For staining of formalin-fixed, paraffin-embedded tissues, digest with trypsin at 1mg/ml PBS, 15 min at RT.); ; Optimal dilution for a specific application should be determined
• Unigene: 112405
• Human Gene Symbol: S100A9
• Hu Gene Location: 1q21
• Immunogen: Human peripheral blood monocyte components
• Cellular Localization: Cytoplasmic, Extracellular (Secreted) and Cell Surface
• Positive Control: Liver or histiocytoma
• Preparation and Storage: Antibody without azide: Store at -20 to -80 °C.; Antibody is stable for 24 months.; Non-hazardous. No MSDS required.

References

Flavell DJ. et al., J. Histochem. Cytochem. 35, 12171226, 1987. Facchetti F. et al., Am. J. Clin. Pathol. 92, 4250, 1989. Bardadin KA. et al., J. Pathol. 164, 253259, 1991. Goebeler M. Et al., J. Leukocyte Biol. 55, 259-261, 1994

NCBI and Uniprot Product Information

• NCBI GI #: 4506773
• NCBI GeneID: 6280
• NCBI Accession #: NP_002956.1
• NCBI GenBank Nucleotide #: NM_002965.3
• UniProt Accession #: P06702; Q6FGA1; Q9NYM0; Q9UCJ1; D3DV36
• Molecular Weight: 14kDa
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9
• NCBI Official Symbol: S100A9
• NCBI Official Synonym Symbols: MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• NCBI Protein Information: protein S100-A9
• UniProt Protein Name: Protein S100-A9
• Protein Family: Protein
• UniProt Gene Name: S100A9
• UniProt Synonym Gene Names: CAGB; CFAG; MRP14; MRP-14; p14
• UniProt Entry Name: S10A9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; signal transducer activity; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; autophagy; caspase activation; cell-cell signaling; chemokine production; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; leukocyte migration during inflammatory response; neutrophil chemotaxis; positive regulation of cell growth; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis; sequestering of zinc ion