Mouse anti-Human S100A9 Monoclonal Antibody | anti-S100A9 antibody

Anti-S100A9 Antibody (PE), Mouse Monoclonal

Cat. Num. AAA8104954

• Gene Names: S100A9; MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: S100A9; Monoclonal Antibody; Anti-S100A9 Antibody (PE); Mouse Monoclonal; S100A9 Antibody (PE); Mouse MAb; S100 calcium binding protein A9; Anti-60B8AG Antibody; Anti-CAGB Antibody; Anti-CFAG Antibody; Anti-CGLB Antibody; Anti-L1AG Antibody; Anti-LIAG Antibody; Anti-MAC387 Antibody; Anti-MIF Antibody; Anti-MRP-14 Antibody; Anti-MRP14 Antibody; Anti-NIF Antibody; Anti-P14 Antibody; anti-S100A9 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 01
• Specificity: Human S100A9
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 1ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-S100A9 antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human S100A9 protein
• Conjugation: PE
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human S100A9 (rh S100A9) and conjugated with PE under optimum conditions, the unreacted PE was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Related Product Information for anti-S100A9 antibody

S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A9, also known as S100 calciumbinding protein A9, S100A9, and CAGB, is a member of the S-100 family. S100A9 is expressed by macrophages in acutely inflammed tissues and in chronic inflammation. It is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

Product Categories/Family for anti-S100A9 antibody

Calcium-binding Proteins and Related Molecules

References

Miyasaki KT. et al., 1993, J Dent Res. 72: 517-23. Fano G. et al., 1995, Prog Neurobiol. 46 (1): 71-82. Vogl T. et al., 2004, Blood. 104: 4260-8. Viemann D. et al., 2005, Blood. 105: 2955-62. Nakatani Y. et al., 2005, Mediators Inflamm. 2005: 280-92. Bjoerk P. et al., 2009, PLoS Biol. 7: E97-E97.

NCBI and Uniprot Product Information

• NCBI GI #: 4506773
• NCBI GeneID: 6280
• NCBI Accession #: NP_002956.1
• NCBI GenBank Nucleotide #: NM_002965.3
• UniProt Accession #: P06702; Q6FGA1; Q9NYM0; Q9UCJ1; D3DV36
• Molecular Weight: 13,242 Da
• NCBI Official Full Name: protein S100-A9
• NCBI Official Synonym Full Names: S100 calcium binding protein A9
• NCBI Official Symbol: S100A9
• NCBI Official Synonym Symbols: MIF; NIF; P14; CAGB; CFAG; CGLB; L1AG; LIAG; MRP14; 60B8AG; MAC387
• NCBI Protein Information: protein S100-A9; MRP-14; S100 calcium-binding protein A9 (calgranulin B); calgranulin-B; calprotectin L1H subunit; leukocyte L1 complex heavy chain; migration inhibitory factor-related protein 14
• UniProt Protein Name: Protein S100-A9
• Protein Family: Protein
• UniProt Gene Name: S100A9
• UniProt Synonym Gene Names: CAGB; CFAG; MRP14; MRP-14; p14
• UniProt Entry Name: S10A9_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014]

Uniprot Description

S100A9: a calcium-binding regulatory protein of the S-100 family expressed by macrophages in acutely inflammated tissues and in chronic inflammations. May be an inhibitor of protein kinases. Also expressed in epithelial cells constitutively or induced during dermatoses. May interact with components of the intermediate filaments in monocytes and epithelial cells. Interacts with CEACAM3 in a calcium-dependent manner.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: extracellular space; cytoskeleton; plasma membrane; extracellular region; cytosol; nucleus

Molecular Function: arachidonic acid binding; antioxidant activity; signal transducer activity; protein binding; RAGE receptor binding; zinc ion binding; microtubule binding; calcium ion binding

Biological Process: caspase activation; neutrophil chemotaxis; chemokine production; chronic inflammatory response; cytokine production; response to lipopolysaccharide; positive regulation of peptide secretion; signal transduction; positive regulation of cell growth; leukocyte migration during inflammatory response; activation of NF-kappaB transcription factor; sequestering of zinc ion; response to ethanol; cell-cell signaling; response to zinc ion; actin cytoskeleton reorganization; defense response to bacterium; autophagy; innate immune response; regulation of integrin biosynthetic process; inflammatory response; defense response to fungus; regulation of cytoskeleton organization and biogenesis; positive regulation of inflammatory response