Mouse S100A8 Monoclonal Antibody | anti-S100A8 antibody

S100A8 Monoclonal Antibody

Cat. Num. AAA9415036

• Gene Names: S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
• Applications: Immunohistochemistry
• Purity: Protein G Purifed
• Synonyms: S100A8; Monoclonal Antibody; S100A8 Monoclonal Antibody; Calgranulin-A; Calprotectin L1L subunit; Cystic fibrosis antigen; CFAG; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 8; MRP-8; p8; S100 calcium-binding protein A8; Urinary stone protein band A; anti-S100A8 antibody

• Host: Mouse
• Clonality: Monoclonal
• Specificity: Specific for human S100A8 denatured and native forms
• Purity/Purification: Protein G Purifed
• Form/Format: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 93

• Applicable Applications for anti-S100A8 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:20 - 1:200
• Immunogen: Recombinant Human S100A8
• Immunogen Type: Protein
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human cervical cancer using at dilution of 1:200.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen using at dilution of 1:200.)

Related Product Information for anti-S100A8 antibody

S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response.It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn2+ which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread.

Product Categories/Family for anti-S100A8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 21614544
• NCBI GeneID: 6279
• NCBI Accession #: NP_002955.2
• NCBI GenBank Nucleotide #: NM_002964.4
• UniProt Accession #: P05109; Q5SY70; Q9UC84; Q9UC92; Q9UCJ0; Q9UCM6; A8K5L3; D3DV37
• Molecular Weight: 10,835 Da
• NCBI Official Full Name: protein S100-A8 isoform d
• NCBI Official Synonym Full Names: S100 calcium binding protein A8
• NCBI Official Symbol: S100A8
• NCBI Official Synonym Symbols: P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
• NCBI Protein Information: protein S100-A8
• UniProt Protein Name: Protein S100-A8
• Protein Family: Protein
• UniProt Gene Name: S100A8
• UniProt Synonym Gene Names: CAGA; CFAG; MRP8; CFAG; MRP-8; p8
• UniProt Entry Name: S10A8_HUMAN

Uniprot Description

S100A8: S100A8 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis and adhesion. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH- oxidase by facilitating the enzyme complex assembly at the cell membrane, transfering arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinfammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF- kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. Can act as a potent amplifier of inflammation in autoimmunity as well as in cancer development and tumor spread. Belongs to the S-100 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytosol; extracellular region; extracellular space; nucleus; plasma membrane

Molecular Function: arachidonic acid binding; calcium ion binding; microtubule binding; protein binding; RAGE receptor binding; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; autophagy; caspase activation; chemokine production; cytokine production; defense response to bacterium; defense response to fungus; inflammatory response; leukocyte migration during inflammatory response; neutrophil chemotaxis; peptidyl-cysteine S-nitrosylation; positive regulation of cell growth; positive regulation of inflammatory response; regulation of cytoskeleton organization and biogenesis; sequestering of zinc ion