Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RuVBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Raji cell lysate )
Mouse anti-Human RuVBL1 Monoclonal Antibody | anti-RuVBL1 antibody
RuVBL1 antibody
Gene Names
RUVBL1; RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
RuVBL1; Monoclonal Antibody; RuVBL1 antibody; RuvB-like 1; ECP54; INO80H; NMP238; PONTIN; Pontin52; RVB1; TIH1; TIP49; TIP49A; RuVBL 1; RuVBL-1; Pontin 52; NMP 238; NMP-238; TIP 49; TIP-49; anti-RuVBL1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT1D6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
456
Applicable Applications for anti-RuVBL1 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
Antigen Species
Human
Immunogen
Recombinant human RuVBL1 (1-456aa) purified from E. coli
Clone Note
Anti-human RuVBL1 mAb, clone AT1D6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human RuVBL1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RuVBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Raji cell lysate )
Western Blot (WB)
(The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RuVBL1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Raji cell lysate )
Immunofluorescence (IF)
(ICC/IF analysis of RuVBL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RuVBL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of RuVBL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human RuVBL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Related Product Information for anti-RuVBL1 antibody
RuVBL1, also known as TIP49A, is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This complex may be required for the activation of transcriptional programs associated with oncogene and proto oncogene mediated growth induction, tumor suppressor mediated growth arrest.
Product Categories/Family for anti-RuVBL1 antibody
References
Jha S., et al. (2009) Mol Cell. 34 (5): 521-33.; Haurie V., et al. (2009) Hepatology. 50 (6): 1871-83.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,127 Da
NCBI Official Full Name
ruvB-like 1 isoform 1
NCBI Official Synonym Full Names
RuvB like AAA ATPase 1
NCBI Official Symbol
RUVBL1
NCBI Official Synonym Symbols
RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52
NCBI Protein Information
ruvB-like 1
UniProt Protein Name
RuvB-like 1
Protein Family
UniProt Gene Name
RUVBL1
UniProt Synonym Gene Names
INO80H; NMP238; TIP49; TIP49A; 49 kDa TBP-interacting protein; ECP-54; NMP 238; TAP54-alpha
NCBI Description
This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Uniprot Description
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity; hexamerization is thought to be critical for ATP hydrolysis and adjacent subunits in the ring-like structure contribute to the ATPase activity.
Research Articles on RuVBL1
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Product Notes
The RuVBL1 ruvbl1 (Catalog #AAA200422) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RuVBL1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RuVBL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the RuVBL1 ruvbl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RuVBL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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