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Western Blot (WB) (RPL13 monoclonal antibody, Western Blot analysis of RPL13 expression in HeLa.)

Mouse anti-Human RPL13 Monoclonal Antibody | anti-RPL13 antibody

RPL13 (60S Ribosomal Protein L13, Breast Basic Conserved Protein 1, BBC1, OK/SW-cl.46, FLJ27453, FLJ27454, MGC117342, MGC71373)

Gene Names
RPL13; L13; BBC1; D16S44E; D16S444E
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
RPL13; Monoclonal Antibody; RPL13 (60S Ribosomal Protein L13; Breast Basic Conserved Protein 1; BBC1; OK/SW-cl.46; FLJ27453; FLJ27454; MGC117342; MGC71373); Anti -RPL13 (60S Ribosomal Protein L13; anti-RPL13 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
3F6
Specificity
Recognizes human RPL13.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
ANVQRLKEYRSKLILFPRKPSAPKKGDSSAEELKLATQLTGPVMPVRNVYKKEKARVITEEEKNFKAFASLRMARANARLFGIRAKRAKEAAEQDVEKKK
Applicable Applications for anti-RPL13 antibody
ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Partial recombinant corresponding to aa112-212, from human RPL13 (NP_000968) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPL13 monoclonal antibody, Western Blot analysis of RPL13 expression in HeLa.)

Western Blot (WB) (RPL13 monoclonal antibody, Western Blot analysis of RPL13 expression in HeLa.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to RPL13 on HeLa cell. [antibody concentration 10ug/ml])

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to RPL13 on HeLa cell. [antibody concentration 10ug/ml])

Testing Data

(Detection limit for recombinant GST tagged RPL13 is ~3ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged RPL13 is ~3ng/ml as a capture antibody.)
Related Product Information for anti-RPL13 antibody
Higher levels of expression in benign breast lesions than in carcinomas.
Product Categories/Family for anti-RPL13 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
24,261 Da
NCBI Official Full Name
RPL13 protein
NCBI Official Synonym Full Names
ribosomal protein L13
NCBI Official Symbol
RPL13
NCBI Official Synonym Symbols
L13; BBC1; D16S44E; D16S444E
NCBI Protein Information
60S ribosomal protein L13; OK/SW-cl.46; breast basic conserved protein 1
UniProt Protein Name
60S ribosomal protein L13
Protein Family
UniProt Gene Name
RPL13
UniProt Synonym Gene Names
BBC1
UniProt Entry Name
RL13_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L13E family of ribosomal proteins. It is located in the cytoplasm. This gene is expressed at significantly higher levels in benign breast lesions than in breast carcinomas. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2011]

Uniprot Description

RPL13: a ribosomal protein. A component of the 60S ribosomal subunit. Reduced expression observed in breast cancer.

Protein type: Translation; Ribosomal

Chromosomal Location of Human Ortholog: 16q24.3|17p11.2

Cellular Component: membrane; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: protein binding; structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; translation; viral reproduction; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; translational termination; viral infectious cycle

Research Articles on RPL13

Similar Products

Product Notes

The RPL13 rpl13 (Catalog #AAA6003323) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RPL13 (60S Ribosomal Protein L13, Breast Basic Conserved Protein 1, BBC1, OK/SW-cl.46, FLJ27453, FLJ27454, MGC117342, MGC71373) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RPL13 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the RPL13 rpl13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ANVQRLKEYR SKLILFPRKP SAPKKGDSSA EELKLATQLT GPVMPVRNVY KKEKARVITE EEKNFKAFAS LRMARANARL FGIRAKRAKE AAEQDVEKKK. It is sometimes possible for the material contained within the vial of "RPL13, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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