Mouse RNPEP Monoclonal Antibody | anti-RNPEP antibody

RNPEP antibody

Cat. Num. AAA833170

• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: RNPEP antibody was purified by affinity chromatography.
• Synonyms: RNPEP; Monoclonal Antibody; RNPEP antibody; Monoclonal RNPEP; Anti-RNPEP; Arginyl aminopeptidase; Aminopeptidase B; AP-B; APB; anti-RNPEP antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2G3
• Purity/Purification: RNPEP antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 650

• Applicable Applications for anti-RNPEP antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC: 1:100; IHC: 1:150; WB: 1:2000
• Biological Significance: RNPEP is an exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptidesubstrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyzeleukotriene A4 (LTA-4) into leukotriene B4 (LTB-4).
• Cross-Reactivity: Human
• Immunogen: RNPEP antibody was raised in mouse using a full length recombinant protein of human RNPEP (NP_064601) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant RNPEP protein (red) or empty vector (blue) stained using RNPEP antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RNPEP protein (Right) or empty vector (Left) detected with RNPEP antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of RNPEP protein in paraffin embedded Adenocarcinoma of Human breast tissue using RNPEP antibody)

Related Product Information for anti-RNPEP antibody

Mouse monoclonal RNPEP antibody

Product Categories/Family for anti-RNPEP antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 40316915
• NCBI GeneID: 6051
• NCBI Accession #: NP_064601
• NCBI GenBank Nucleotide #: NM_020216.3
• UniProt Accession #: Q9H4A4; Q9BVM9; Q9H1D4; Q9NPT7
• Molecular Weight: 72,596 Da
• NCBI Official Full Name: aminopeptidase B
• NCBI Official Synonym Full Names: arginyl aminopeptidase (aminopeptidase B)
• NCBI Official Symbol: RNPEP
• NCBI Protein Information: aminopeptidase B
• UniProt Protein Name: Aminopeptidase B
• Protein Family: Aminopeptidase
• UniProt Gene Name: RNPEP
• UniProt Synonym Gene Names: APB; AP-B
• UniProt Entry Name: AMPB_HUMAN

Uniprot Description

RNPEP: Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(- 1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4). Belongs to the peptidase M1 family.

Protein type: EC 3.4.11.6; Protease

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: metalloexopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; proteolysis