Mouse RNA Polymerase II Monoclonal Antibody | anti-POLR2E antibody

RNA Polymerase II Monoclonal Antibody

Cat. Num. AAA190407

• Gene Names: POLR2E; RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• Synonyms: RNA Polymerase II; Monoclonal Antibody; RNA Polymerase II Monoclonal Antibody; Anti-RNA Polymerase II; anti-POLR2E antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: Anti-RNA Polymerase II [Murine MAb recognizes the highly conserved heptapeptide repeat of the largest subunit of eukaryotic RNA polymerase II.]; This antibody reacts with the highly conserved heptapeptide repeat of the largest subunit of eukaryotic RNA polymerase II. It inhibits promoterdirected transcription, but it does not inhibit elongation in the nonspecific transcription assay.
• Form/Format: 200 ug Protein G purified antibody in PBS, pH 7.4.
• Sequence Length: 210

• Application Notes: This antibody may be used to detect unproteolyzed RNA polymerase II or to inhibit specific transcription from class II promoters. This is a polyol-responsive antibody and can be used in gentle purifications of RNA polymerase II from from wheat germ, calf thymus, and yeast and is likely to purify RNA polymerase II from most eukaryotic organisms.
• Antigen: Purified wheat germ RNA polymerase II.
• Preservatives: None. Available on request
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: These antibodies are stable for at least one (1) year at -20 degree C to -70 degree C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles

Product Categories/Family for anti-POLR2E antibody

Protein Transcription Antibodies

References

Thompson NE et al. 1989. J Biol Chem 264: 11511-11520; Thompson NE et al. 1990. J Biol Chem 265: 7069-7077.

NCBI and Uniprot Product Information

• NCBI GI #: 232954
• NCBI GeneID: 5434
• UniProt Accession #: P19388; O43380; Q6PIH5; Q9BT06; B2R6L4; D6W5Y1
• Molecular Weight: 24,551 Da
• NCBI Official Full Name: RNA polymerase II
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• NCBI Official Symbol: POLR2E
• NCBI Official Synonym Symbols: RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC1; RPB5 homolog; DNA-directed RNA polymerase II subunit E; RNA polymerases I, II, and III subunit ABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• Protein Family: RNA polymerase II assembly factor
• UniProt Gene Name: POLR2E
• UniProt Entry Name: RPAB1_HUMAN

NCBI Description

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]

Uniprot Description

POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; cytosol; nucleus; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; somatic stem cell maintenance; positive regulation of viral transcription; RNA splicing; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; nuclear mRNA splicing, via spliceosome; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter